Unit 1- Lab techniques

Question 1

What are two precautions when working with chemicals

Answer 1

Wear gloves and a lab coat
Ensure it is disposed safely
Have an established procedure for spills
Maintain a clean working area before and after use

Question 2

What is PPE

Answer 2

Personal protective equipment

Question 3

Why is PPE used?

Answer 3

To protect the body from harm

Question 4

Give an example of PPE which would be appropriate for working with UV light

Answer 4

A lab coat
Safety glasses
A face shield

Question 5

Why are buffers used?

Answer 5

To keep PH at a constant value

Question 6

Name a piece of equipment that is used to measure the absorbance of a solution

Answer 6

Colorimeter

Question 7

Which type of cell in the mouse produces antibodies

Answer 7

B lymphocytes

Question 8

What term is used to describe fused cells

Answer 8

Hybridomas

Question 9

Name the chemical used to make the cells fuse together

Answer 9

PEG/ polyethylene glycol

Question 10

What substance, containing growth factors, must be added to culture media to allow successful growth of animal cells in culture

Answer 10

Serum

Question 11

Why is it important to follow aseptic techniques when carrying out cell culture

Answer 11

To prevent contamination

Question 12

Other than wearing gloves, a lab coat, name one other aseptic technique

Answer 12

Wiping down surface with ethanol( disinfectant)
Working in a cell culture hood
Washing hands before and after a procedure
Using sterile equipment

Question 13

Scientist often need ascertain the number of cells in their culture. Other than a microscope, what piece of equipment is required to perform a cell count

Answer 13

Haemocytometer

Question 14

What is a dilution series?

Answer 14

Repeated solution from a stock solution

Question 15

How is a dilution series performed?

Answer 15

Icm3 stock solution into a test tube with 9cm3 water=10-1

Question 16

Describe one use of a dilution series

Answer 16

If there are too many cells to count you can use a dilution series

Question 17

What is a colorimeter

Answer 17

A deuce used to measure the absorbance of a specific wavelength of a light by a solution

Question 18

How is a colorimeter used?

Answer 18

It works by passing a light beam, at a specific wavelength, through a curvette containing a specific solution

Question 19

Give an example of a buffer found in the human body

Answer 19

Carbonic acid to mai rain PH of blood

Question 20

What name is given to the test which allows to the concentration of protein in a solution to be determined?

Answer 20

Bradford protein assy

Question 21

What is a centrifuge?

Answer 21

A centrifuge is a piece of equipment that spins on object at High speed

Question 22

How can proteins be separated?

Answer 22

Gel electrophoresis

Question 23

How does the process work?

Answer 23

Proteins travel towards the electrode with an opposite charge, smaller proteins will travel further through the gel than larger proteins

Question 24

What does the gel act as In gel electrophoresis

Answer 24

A sieve which the proteins move in response to the electric field

Question 25

What Is paper chromatography?

Answer 25

Using paper as a stationary phase and using solvent as mobile phase