Unit 1- Lab techniques Flashcards
What are two precautions when working with chemicals
Wear gloves and a lab coat
Ensure it is disposed safely
Have an established procedure for spills
Maintain a clean working area before and after use
What is PPE
Personal protective equipment
Why is PPE used?
To protect the body from harm
Give an example of PPE which would be appropriate for working with UV light
A lab coat
Safety glasses
A face shield
Why are buffers used?
To keep PH at a constant value
Name a piece of equipment that is used to measure the absorbance of a solution
Colorimeter
Which type of cell in the mouse produces antibodies
B lymphocytes
What term is used to describe fused cells
Hybridomas
Name the chemical used to make the cells fuse together
PEG/ polyethylene glycol
What substance, containing growth factors, must be added to culture media to allow successful growth of animal cells in culture
Serum
Why is it important to follow aseptic techniques when carrying out cell culture
To prevent contamination
Other than wearing gloves, a lab coat, name one other aseptic technique
Wiping down surface with ethanol( disinfectant)
Working in a cell culture hood
Washing hands before and after a procedure
Using sterile equipment
Scientist often need ascertain the number of cells in their culture. Other than a microscope, what piece of equipment is required to perform a cell count
Haemocytometer
What is a dilution series?
Repeated solution from a stock solution
How is a dilution series performed?
Icm3 stock solution into a test tube with 9cm3 water=10-1
Describe one use of a dilution series
If there are too many cells to count you can use a dilution series
What is a colorimeter
A deuce used to measure the absorbance of a specific wavelength of a light by a solution
How is a colorimeter used?
It works by passing a light beam, at a specific wavelength, through a curvette containing a specific solution
Give an example of a buffer found in the human body
Carbonic acid to mai rain PH of blood
What name is given to the test which allows to the concentration of protein in a solution to be determined?
Bradford protein assy
What is a centrifuge?
A centrifuge is a piece of equipment that spins on object at High speed
How can proteins be separated?
Gel electrophoresis
How does the process work?
Proteins travel towards the electrode with an opposite charge, smaller proteins will travel further through the gel than larger proteins
What does the gel act as In gel electrophoresis
A sieve which the proteins move in response to the electric field
What Is paper chromatography?
Using paper as a stationary phase and using solvent as mobile phase
