Unit 1 - Cells and Protein Flashcards

1
Q

Eukaryotic cells have large/ small surface area:volume

A

Small due to size

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2
Q

What do eukaryotic cells provide to increase surface area

A

System of internal membranes so vital functions can take place

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3
Q

Full name of ER

A

Endoplasmic reticulum

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4
Q

What is the ER

A

Membrane network continuous of nuclear membrane

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5
Q

What is the cytosolic face

A

Side that faces cytoplasm

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6
Q

Difference between rough ER and smooth ER

A

rough ER has ribosomes on cytosolic face - smooth ER doesnt

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7
Q

What is the Golgi Apparatus

A

Series of flattened membrane discs

  • connected to allow mollecules to move along GA
  • adjacent ER
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8
Q

Define vesicle

A

Transport material between membrane compartments

-consist of aqueous solution enclosed by lipid bilayer

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9
Q

Definie secretory vesicle

A

Vesicle that transports hormones ect. from organelles to specific sites at cell membrane where it docks on and releases content

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10
Q

What are Lysosomes

A

Membrane bound organelles containing variety of hydrolases

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11
Q

What do hydrolases do

A

Digest protein, lipids, nucleic acid and carbohydrates

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12
Q

What is the interior of a Lysosome like

A

Acidic - allows optimal enzyme function

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13
Q

What is a signal sequence

A

Short strech of amino acids at one end of the polypeptide chain that determines location of protein in cell

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14
Q

What are covalent modifications

A

Enzyme calatlysed alterations of synthesised proteins

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15
Q

What do covalent modifications include

A

Addition or removal of chemical groups - can target single thpe of amino acid or myltiple amino acids and will change the chemical properties of the site

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16
Q

When does post translational modification take place

A

As molecules move through Golgi Disks in vesicle thay bud off from one disc and fuse to next

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17
Q

Post translational modifications (8)

A

Phosphorylation, Methylation, Lipidation, Acetylation, SuMoylation, Ubiquitination, Glycosylation, Hydroxylation

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18
Q

What is Phosphorylation

A

Addition of Phosphate to serine/threonine/thyrosin

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19
Q

What is Methylation

A

Addition of CH4 group usually to Lysine/ arginine Vesicle

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20
Q

What is Lipidation

A

Attachment of lipid/ fatty acid to protein chain

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21
Q

What is Acetylation

A

Addition of Acetyl group to N-terminus of protein/ Lysine residue

22
Q

What is SuMo

A

Small ubiquitin like modifier

23
Q

What is SuMoylation

A

Addition of SuMo to target protein

24
Q

What is ubiquitination

A

Addition of ubiquitin to Lysine residue of target protein of degredation

25
What is libiquitin
Small protein
26
What is glycolysation
Attachment of sugar usually to N or O in amino acid side chain
27
What is hydroxylation
Attachment of -OH group to side chain of protein
28
What are microtubules
Hollow, fibrous shafts that support give cell shape
29
What is a secretory protein
Any protein secreted by a cell
30
What are 2 examples of secretory proteins
Hormones (eg insulin), emzymes (eg trypsin)
31
Where are secretory proteins translated
In ribosome on RER
32
Difference in secretory vesicles and vesicles (mobility)
Secretory vesicles- transport cell secretion = can move | Vesicles- membrane bound = cant move
33
What does precursor stand for
Parent
34
Many secretory vesicles are synthesised and (1) and require (2) to produce (3)
1 inactive precursors 2 proteolytic cleavage 3 active protein
35
What is proteolytic cleave
A type of posttranslational modification eg digestive proteins
36
What are amino acids and what do they do
Building blocks of protein | - determine structure
37
Whats a monomer
Molecule that may bind to other molecules to form polymer
38
What is a polymer
Large molecule made of subunits (monomers)
39
Proteins are monomers/polymers
Polymers
40
What type of bonds links amino acids
Peptide bonds
41
What type of binds are peptide bonds
Covalent bonds
42
Amino group
H3N
43
Amino acids have same (1) but (2) may differ
1 basic structure | 2 R- Groups
44
Types of amino acids
Acidic/ basic/ polar/ non polar
45
Properties of acidic amino acids
Negatively charged - hydrophilic - COO- - pH 7
46
Properties of basic amino acids
Positively charged - hydrophilic - NH3 - pH7
47
Properties of polar amino acids
- hydrophilic | - C=H/ CH/ NH
48
Properties of non polar amino acids
- hydrophobic | - hydrocarbon group
49
Low pH = (1) | High pH = (2)
1 pos charge | 2 neg charge
50
Define Isoelectric point
pH at which net charge is 0
51
How does isoelectric point affect amino acids
They are least soluble at isoelectric point