Unit 1 Flashcards
What are animal cell membranes composed of?
Protein and lipid
What is diffusion ?
The passive movement of molecules from a region of high concentration to low concentration until evenly spread
What is Osmosis?
The passive movement of water mile clues from a region of high water concentration to low water concentration through a selectively permeable membrane
What is active transport ?
The movement of molecules from a region of low concentration to s higher concentration against s concentration gradient
Requires energy
Examples of active transport
Sodium potassium pump in nerve cells
Potassium ions in nitella
Example of osmosis
Uptake of water into roots of plants
Example of diffusion
Respiration
Animal cell in water
HWC?
LWC?
Equal ?
HWC- burst
LWC- shrink
Equal - no change
Plant cell in water
HWC?
LWC?
Equal ?
HWC- turgid
LWC- plasmolysed flaccid
Equal- no change
Diploid - humans
46
Haploid - humans
23
How is an organism formed ?
DNA Gene Chromosome Nucleus Cell
Why do we use Aseptic technique?
To avoid contamination
Factors that effect aseptic technique
Temperature
PH
Oxygen levels
Name the 4 DNA bases
Adenine
Thymine
Cytosine
Guanine
In mRNA what replaces thymine ?
Uracil
Role of DNA
Genetic information
Describe the shape of a DNA molecule
Double stranded helix
Functions of proteins
Enzymes Structure Hormones Antibodies Receptors
Examples of enzymes and their pH
Pepsin - pH2
Catalyse - pH9
Examples of GM organisms
Tomato- increase yield
Potato- fungal resistance and do increasing yield
Photosynthesis equation
Light
Water+CO2—————>glucose+O2
Chlorophyll
what are the Two stages of photosynthesis ?
Light dependant
Carbon fixation
Photosynthesis diagram
Light energy
⬇️
⬇️ ⬇️
Water Chlorophyll
⬇️ ⬇️ ⬇️
Hydrogen O2 Energy
⬇️
⬇️ ↪️ATP–ADP+phosphate↩️
⬇️ ⬇️
⬇️
Hydrogen+ carbon dioxide–>glucose
Test for starch
Iodine solution turns from Brown to black /blue
Limiting factors for photosynthesis
Light intensity
Temperature
Concentration of CO2
ATP wheel diagram
Respiration
CO2+water
Energy ⤴️
Glucose+oxygen
What are plant cell walls composed of?
Cellulose
ATP wheel
Energy transfer
ATP
Energyenergy
ADP+Pi
ATP wheel
Cellular processes
Relaxed muscles
Energy↪️
Contracted muscle
Mitosis stage four
Chromatids pulled to north and South poles
Mitosis stage five
Nuclear membranes form and cytoplasm splits to form two daughter cells
Cell culture step 1
Metal loop heated strongly to sterilise it
Cell culture step 2
Sterile loop used to pick up bacteria of a pure colony from an agar plate
Cell culture step 3
Bottle lid removed and mouth of bottle sterilised by passing it through flame - bacteria transferred to sterile liquid agar in bottle using loop
Cell culture step 4
Bottle mouth sterilised by passing through a flame then lid replaced tightly
Cell culture step 5
Loop finally sterilised
Genetic engineering step 1
Insulin gene located in chromosome using gene probe
Genetic engineering step 2
Insulin gene removed from chromosome using endonuclease enzymes
Plasmid removed from bacterial cell and cut open using endonuclease
Genetic engineering step 3
Human insulin gene sealed into plasmid using ligase enzyme
Genetic engineering step 4
Genetically engineered bacterial cell
Hybrid plasmid introduced into bacterial cell
Bacteria cultured and human insulin issued and purified
Mitosis stage three
Chromosomes line up at the equator
Mitosis stage one
Cell with diploid number of four
Mitosis stage two
Chromosomes replicate and spindle Appears