UNIT 1 Flashcards

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1
Q

Pros of light microscopy

A
  • used to see live and whole cells, can use color, track cells, cheap and easy to use
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2
Q

Resolution limit of light microscopy

A

200 nm (smaller structures cannot be seen)

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3
Q

Organelles that can be seen in brightfield

A

nucleus, mitochondria, chloroplasts, vacuoles, plasma membrane, cytoplasm

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4
Q

Purpose of fluorescence microscopy

A

localize molecules or structures in cells by using fluorescent markers

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5
Q

Organelles that can be seen in fluorescence

A

nucleus (binds DNA), membrane, microtubules mitochondria, endoplasmic reticulum, golgi apparatus, cytoskeleton and lysosomes

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6
Q

Immunofluorescence

A
  1. localizes proteins of interest with primary antibodies.
  2. secondary antibodies covalently linked to fluorescent molecules recognize the primary antibody and amplifies the signal
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7
Q

Clues that tell you the type of microscopy that was used?

A
  • check background (is it black)
  • how much detail it shows
  • what organelles are shown?
  • scale?
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8
Q

Cons of light microscopy

A
  • resolution is 200nm
  • we cannot see smaller structures
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9
Q

When can we use transmitted microscopy (eg brightfield)

A

when we don’t need color and to see whole tissues

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10
Q

When can we use fluorescence microscopy?

A

when we want to see the localization of protein of interest, if we need to see color, track proteins or organelles

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11
Q

Resolution of Electron microscopy

A

Resolution limit is 0.2 nm

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12
Q

SEM pros

A
  • can see 3D images
  • high resolution
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13
Q

Disadvantages of SEM

A
  • cells must be dead
  • complex specimen preparation
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14
Q

TEM pros

A
  • details of cytoplasm can be seen
  • high resolution
  • can see detailed internal structures
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15
Q

Disadvantages of TEM

A
  • dead cells
  • complex specimen prep
  • 2D images
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16
Q

Organelles that can be seen in SEM

A

cell membrane and surface structures (eg cilia)

17
Q

Organelles that can be seen in TEM

A

Nucleus, mitochondria (with detailed cristae), ribosomes, endoplasmic reticulum, Golgi apparatus, cytoskeleton structures (microtubules, microfilaments), lysosomes, peroxisomes.