Unit 1

Question 1

Which model has RNAi?

Answer 1

C. Elegans

Question 2

Which model has polytene chromosomes

Answer 2

Flies

Question 3

Common protein tags

Answer 3

Flag
Myc
His
GFP

Question 4

SDS

Answer 4

Negative charge detergent

Question 5

BME

Answer 5

Used in PAGE. Breaks SS linkages

Question 6

Phosphorylation can happen on

Answer 6

Serine threonine tyrosine

Question 7

Monoubiquitination for

Answer 7

Histone regulation

Question 8

Multiubiquinationgl for

Answer 8

Endocytosis

Question 9

Polyubiquinstion for

Answer 9

Proteosomal degradation
DNA repair

Question 10

What eats the primer?

Answer 10

RNAse H

Question 11

What do single strand dna binding proteins do

Answer 11

Straighten for polymerase (don’t touch bases)
Help helicase

Question 12

How to telomeres not get degraded

Answer 12

T-loops

Question 13

What happens in depurination

Answer 13

Abasic site, deletion

Question 14

What happens in deaminatiin

Answer 14

Cytosine becomes uracil, pairs with A, substitution

Question 15

Describe base excision repair

Answer 15

DNA glycosylases recognize and flip out unnatural base, cleave glycosidic bond. AP endonycleaze and phosphodiesterase then cute the sugar-phosphate,

Question 16

Inscribe nucleotide excision repair

Answer 16

Removed bulky lesions like py diners and hydrocarbons
Multienxyme complex scans dna, cleaves both ends
Helicase peels away ssDNA

Question 17

HR mechanism

Answer 17

Nuclear digests 5’ ends
Strands exchanged via complementary base pairing
Repair polymerase

Question 18

Difference about HR in meiosis?

Answer 18

Programmed DSB
gene conversion and crossing over

Question 19

Problem with NHEJ?

Answer 19

Changes sequence