Unit 1 Flashcards
What is an assay
process of determining the amount of an analyte in a sample
Signal to Noise Ratio Eq
S/N= k sqrt(number of measurements)
What is a sample matrix/blank
Sample with everything but analyte
Limit of Detection
Signal= Blank +3(standard deviation of blank)
Limit of Detection
Signal= Blank +10(standard deviation of blank)
Positive control
Prevents false negative results/ will always show the signal
Negative Control
Prevents false positive results/ will never show the signal
Selectivity
How much does interferences impact it
Systematic vs Random error
Systematic is reproducible random is not
Standard Addition
Use slope of line to extrapolate the x-intercept
Standard Addition benefit
Accounts for matrix effects
Internal Standards
Use a compound that behaves similar to the analyte
Internal Standard Equation
Sa/Ca= k Si/Ci (k=response factor)
Internal Standards benefit
Accounts for experimental loss
What percent of Gaussian is within 1 standard deviation
68.3
What percent of Gaussian is within 2 standard deviation
95.5
What percent of Gaussian is within 3 standard deviation
99.7
Case 1 T-Test
Experimental vs Measured value
Case 2 T-Test
Experimental vs Experimental (different samples)
Case 3 T-Test
Experimental vs Experimental (SAME samples)
