Unit 1

Question 1

Which of the following are components of an exposure control plan?
a. Safety education, standard precautions, PPE, disposal of materials
b. Safety education, engineering controls, PPE, quality control
C. Safety educations, standard precautions, PPE, lab blueprint
d. Standard precautions, PPE, education, antibiotic stewardship

Answer 1

a. Safety education, standard precautions, PPE, disposal of materials

Question 2

Selection of media to inoculate for any given specimen is usually based on:
a. the characteristics of the organisms most likely to be involved in the disease
process.
b. the likelihood and types of normal flora present at the site.
C. the most likely pathogens of the suspected disease.
d. All of the above are true.

Answer 2

d. All of the above are true.

Question 3

Which type of media paired with the correct media example is designed to support the
growth of a wide range of microorganisms?
a. Selective- Blood
b. Enriched - Chocolate
C. Differential- Columbia
d. Definitive- Salmonella/Shigella (SS)

Answer 3

b. Enriched - Chocolate

Question 4

Which type of media paired with the correct media example is designed to distinguish
microorganisms on the basis of certain growth characteristics?
a. Selective - Salmonella/Shigella (SS)
b. Enriched- Blood
c. Differential - Hektoen
d. Definitive- Salmonella/Shigella (SS)

Answer 4

c. Differential - Hektoen

Question 5

Which type of media paired with the correct media example is designed to support the
growth of one group of organisms, but NOT another, by containing antimicrobials, dyes,
or alcohol?
a. Selective - Columbia (CNA)
b. Enriched- Blood
c. Differential- Salmonella/Shigella (SS)
d. Definitive- MacConkey

Answer 5

d. Definitive- MacConkey

Question 6

The beta-hemolytic bacteria that is sensitive to Bacitracin is the primary pathogen seen
in what type of sample?
a. Nasopharyngeal swab
b. Urine
C. Abscess
d. Throat swab

Answer 6

d. Throat swab

Question 7

Colonial characteristics that should be examined includes all of the following, EXCEPT:
a. size
b. pigment
C. shape
d. distance

Answer 7

d. distance

Question 8

. The slide coagulase test is a rapid screening test for the production of:
a. bound coagulase.
b. free coagulase.
C. extracellular coagulase.
d. coagulase enzyme.

Answer 8

a. bound coagulase.

Question 9

. Indole production, methyl red, Voges-Proskauer, citrate, urease, oxidase, H2S
production, and gelatin liquefaction are tests historically performed on a
a. gram-negative bacillus.
b. gram-negative coccus.
c. gram-positive coccus.
d. gram-positive bacillus.
e. All of the above are correct.

Answer 9

a. gram-negative bacillus.

Question 10

Direct examinations such as the Gram stain serve what purpose?
a. To assess the quality of the specimen
b. To give the physician an early indication of an organism
C. To guide the workup of the specimen
d. All of the above are correct.

Answer 10

d. All of the above are correct.

Question 11

While performing the quality control on a Gram stain procedure using a control slide of
Staphylococcus and E. coli, the laboratory professional notices that all of the bacteria are
staining pink. This may be due to:
a. Insufficient decolorization
b. Insufficient staining with the primary stain
c. Failure to add acid alcohol
d. Failure to add safranin

Answer 11

b. Insufficient staining with the primary stain

Question 12

Gram positive bacteria retain the primary stain because of the:
a. high content of peptidoglycan in the cell wall
b. low content of peptidoglycan in the cell wall
C. high amount of lipopolysaccharide in the cell wall
d. mycolic acid and waxy compounds in the cell wall

Answer 12

a. high content of peptidoglycan in the cell wall

Question 13

Which of the following is correct for acute and convalescent phase specimens?
a. The convalescent phase specimen must have a lower amount of antibody than the acute phase specimen.
b. The acute specimen is collected one week after the patient displays symptoms.
c. A four-fold increase between the acute and convalescent phase specimens is
diagnostic.
d. Any antibody titer is diagnostic for infection.

Answer 13

c. A four-fold increase between the acute and convalescent phase specimens is
diagnostic.

Question 14

In indirect agglutination techniques:
A. the antibody is detected
B. carrier particles are used n
C. latex beads can be a marker
D. all of these are correct

Answer 14

D. all of these are correct

Question 15

What two types of cells are evaluated on a sputum gram stain for acceptability? What
would constitute an unacceptable sputum sample?

Answer 15

Epi’s and neutrophils. Unacceptable = >10 epi’s/lpf and <25 neutrophils/lpf