UNDERSTAND THIS BY TMR!!!!!! P2

Question 1

Define: Hydrolytic Enzymes

Answer 1

Also known as Hydrolases, are proteins that break down other molecules into smaller fragments

Question 2

Hydrolysis

Answer 2

A chemical reaction that involves adding water to break apart molecules

Question 3

Extracellular Enzymes

Answer 3

A catalyst that is discharged and acts on the outer part of the cell

Question 4

What is the purpose of extracellular enzymes?

Answer 4

Break down complex organic matter into smaller units that can be used by the cell

Question 5

What enzyme breaks down starch?

Answer 5

Amylase

Question 6

What media is used to detect starch hydrolysis?

Answer 6

Starch agar

Question 7

What reagent is used to detect the presence of starch?

Answer 7

Iodine/ Iodine-KI

Question 8

If starch has been hydrolyzed, what results are observed?

Answer 8

Depending on how long it’s allowed to react if the starch is hydrolyzed completely, result product is glucose

Question 9

Define Casein

Answer 9

A protein found in milk and dairy products

Question 10

What enzyme breaks down casein

Answer 10

Rennin, also known as chymosin, breaks down casein

Question 11

What media is used to detect casein hydrolysis?

Answer 11

Skim milk agar

Question 12

If casein has been hydrolyzed, what results are observed?

Answer 12

A clear area forms around the growth, which is a positive result

Question 13

Define gelatin

Answer 13

Is colorless, odorless, flavorless protein substance that comes from animal collagen

Question 14

What enzyme breaks down gelatin?

Answer 14

Bromelain which is a protease breaks down gelatin

Question 15

What media is used to detect gelatin hydrolysis?

Answer 15

A nutrient gelatin medium

Question 16

If gelatin has been hydrolyzed, what results are observed?

Answer 16

A liquid medium and smaller peptides and amino acids

Question 17

What enzyme breaks down lipids?

Answer 17

Lipase

Question 18

What media is used to detect lipid hydrolysis?

Answer 18

Tributyrin agar

Question 19

If lipid has been hydrolyzed, what results are observed?

Answer 19

Produces glycerol and fatty acids

Question 20

Define hemolysin

Answer 20

A substance that causes the destruction of red blood cells

Question 21

Beta hemolysis

Answer 21

A complete lysis of red cells in the media around and under the colonies

Question 22

Alpha hemolysis

Answer 22

A partial breakdown of red blood cells

Question 23

Gamma hemolysis

Answer 23

Gamma indicates the lack of hemolysis, no reaction in the surrounding