U 5 Flashcards
Steps of tissue prep
Fixation
Sectioning
Staining
Four basic types of tissue
Epithelial
Connective
Muscular
Nervous
A matrix formed by the basal lamina and reticular lamina
Basement membrane
Single layer of stem cells in the layers of the epidermis
Stratum basale
Mitotically active prickle cell layer
Stratum spinosum
Non proliferating cells with stained granules
Stratum granulosum
In cross section: a transparent layer seen only on thick skin
Stratum lucidum
Keratinized cell ‘ghosts’
Stratum corneum
Micovilli covered surface of simple cuboidal epithelium and simple columnar epithelium. Especially in small intestine and kidney
Brush boarder (or striated boarder)
Stains cytoplasm, cytoplasmic filaments, collagen fibers, and basement membrane
Objects stained are “acidophilic”
Eosin dye
Stains heterchromatin, nucleolus, the rough ER and sulfonated GAGs
Objects stained are said to be basophilic
Hematoxylin dye
Proliferation of cells that may result in the gross enlargement of an organ
Hyperplasia
Functions of skin
Barrier Sensory Immune Homeostasis Endocrine and exocrine
These dendritic cells take up an process microbial antigens and present them during a skin infection
Skin associated lymphoid tissue
Langerhans cells
Oval receptor cells found in the skin that synapse with somatosensory afferents
Mainly Found in stratum basale
Merkel cells
Pink dye
Eosin
Blue dye
Hematoxylin
Longer than typical microvilli
Common in male reproductive system
Stereocilia
Microvilli covered surface of simple cuboidal + columnar epithelium
Common in intestines
Striated boarder
(Microvilli/cilia) are tail like projections that can be motile or non-motile
Cilia are small tail like projections
(Microvilli/cilia) are cell membrane protrusions that increase surface are
Microvilli are larger cell membrane protrusions
Increase in the number of cells or proliferation of cells
Hyperplasia
The increase in the volume of an organ or tissue due to the enlargement of its component cells
Hypertrophy
Two characteristics that make thick skin distinguished from thin skin
Striatum lucidum and thick stratum corneum (keratinized layer)
Abnormal replacement of one type of tissue with a different type
Metaplasia
Keratohyaline granules are synthesized in the (basal layer/spinous layer)
Spinous layer
Dense, non membrane bound masses of filaggrin and other proteins
Keratohyaline granules
Melanocytes are located in the (stratum basale/stratum spinosum)
Stratum basal
Three types of cartilage
Hyaline
Fibrocartilage
Elastic
Consequences of avascular cartilage
Size limitation
Low metabolic rate
Poor potential for repair
Treatment with systemic drugs difficult
Neuro vascular canals that run parallel to the long axis of a bone
Haversion canals
Connective tissue develops from embryonic mesoderm and, in the head, from the neural crest migration of ______ cells
Mesenchymal
Glandular simple columnar epithelial cell who’s function is to produce gel forming mucins, the major component of mucus
Goblet cell
Simple columnar epithelial cells found in the small intestine, Colin and appendix
Enterocytes
Layer of skin between the epidermis and subcutaneous tissue
Dermis
Synthesize the ECM including connective tissue fibers
Fibroblasts
Three types of (protein) fibers of the ECM
Reticular
Elastic
Collagen
Four types of resident cells
Mesenchymal Reticular cells Adipocyte Fibroblasts (fibrocytes)
Most common connective tissue cell
Fibroblasts
Most easily identified when tissue is stained with trypan blue while animal is alive
Macrophage
Leave bone marrow as B lymphocytes, then differentiate as _______ commonly in lymph nodes
Plasma cells
Have granules containing heparin, chomdroitin sulfate, and histamine.
Known for their role in allergy and anaphylaxis
Mast cells
Cells often found around venules
Mast cells
Primary producers of circulating antibodies (immunoglobulin)
Plasma cells
_____ fiber allows stretch and recoil of blood vessels
Elastic
Composed of type III collagen
Reticular fibers
Composed of type I collagen
Collagen fibers
_____ fibers create flexible networks in lymph nodes, the liver, red bone marrow and spleen.
Reticular fiber
Three basic components of all connective tissue
Cells
Fibers
Ground substance
Transparent, colorless mixture that binds cells to connective tissue. Made of glycosaminoglycans (GAG)
Ground Substance
Connective tissue in the body is derived from which embryonic germ layer
Mesoderm
Connective tissue of the head is derived from which embryonic cells
Neural crest
Ehlers-Danlos type IV disease associated with type ____ collagen in _____ fibers
Type III collagen
Reticular Fibers
Mutations in the fibrillin gene results in ______ where patients are at risk for aortic rupture and hyper mobile joints
Marfan’s syndrome
_____ fibers stain black with silver
Reticular
Principle GAGs in cartilage
Chondroitin 4-sulfate
Chondroitin 6-sulfate
Keratan sulfate
GAG + core protein = ?
Proteoglycans
Cartilage at AC joints, costal, nose, larynx, trachea, bronchi, epiphyseal plate
Hyaline Cartilage
Cartilage at intervertebral discs, pubic symphysis, menisci, some tendons, menisci of knee joint
Fibrocartilage
Cartilage of external ear, epiglottis, Eustachian tube, several laryngeal cartilages.
Elastic cartilage
Chondrocytes deep in the cartilage in groups of up to 8 that originate from a single chondrocyte
Isogenous aggregate
Consists of collagen type I and fibroblasts and surrounds bones
Perichondrium
In the Perichondrium the outer layer contains ______ cells while the inner layer contains _______ cells
Fibrocytes
Chondrocytes
The Perichondrium consists of an outer _____ layer and an inner ______ layer
Outer fibrous layer
Inner chondrogenic layer
______ cartilage stains specifically with orcein dyes
Elastic cartilage
_____ cartilage is at the menisci of the knee joint
Fibrocartilage
______ cartilage is most susceptible to calcification, which is common during aging
Hyaline
Most of the cartilage of Fibrocartilage is type _____
Type 1
Spongy bone is often called _____ or ______
Cancellous or trabecular bone
Compact bone is sometimes called _____ bone
Cortical bone
Both chondrocyte and osteocytes reside in spaces in the matrix called ______
Lacunae
70 percent of bone consists of ______, a _____ phosphate mineral
Hydroxyapatite, a calcium phosphate
_____ bone has no Haversian canals
Spongy, cancellous, trabecular
Osteocytes of spongy bone is (identical/different) from those of compact bone
Identical
Bone ______ are microscopic canals between the lacunae of ossified bone
Canaliculi
The _____ of osteocytes extend into the Canaliculi
Processes
(Spongy/compact) bone is more responsive to changes in load than (Spongy/compact) bone.
(Spongy) bone is more responsive to changes in load than (compact) bone.
The _____ of osteocytes function as the mechanotransducers of local strain in bone
processes
Osteocytes exchanges nutrients and waste at ____ junctions
Gap junctions
The structural unit of compact bone is called an _______
Osteon
The rounded end of a long bone is called an _______
Epiphysis
Cylindrical units that run parallel to the long axis of compact bone
Haversian canals
(Haversian/volkman’s) canals run at oblique angles to the long axis of bone
Volkman’s
_____ covers the internal surface of bones
Endosteum
The middle portion of a long bone is called the
Diaphysis
Most bone is organized as _____ bone characterized by multiple layers of calcified matrix
Lamellar
In each lamella, ______ fibers are aligned in parallel, with the pitch of the fibers orientation shifting orthogonally with each successive lamellae
Type 1 collagen fibers
The process by which bone is constantly being broken down and replaced
Remodeling (or functional adaptation)
During bone resorption, osteoclasts form a special ______ that facilitates the removal of bone
Ruffled boarder
Types of bone cells:
_____ are star shaped cells that live in bones
_____ make bone
_____ reabsorb bone
Osteocytes
Osteoblasts
Osteoclasts