Types of Microscope Preparations and Types of Microtomes Flashcards
Size of a microscope slide
75 x 26 mm (3 x 1 inch)
Used to hold objects under a microscope
Microscope Slide
The type of microscope slide that has round indentations/depressions in the slide
Concave Style
The type of microscope slide that is flat and smooth on the surface
Flat Style
Microscope slide that can be stored for a long time
Permanent Slide
Few trapped air bubbles, less chance of sample loss
Permanent Slide
Two types of temporary slides
Wet mount and Dry mount
Made for short time observations
Temporary Slides
Usually uses liquids, thus is discarded after use
Temporary Slide
Uses liquids like water, glycerin, and oil immersion as a medium
Wet mount slides
A liquid that has preserving characteristics
Glycerine
Does not need a mounting medium
Dry Mount slide
Places a cover slip
Dry mount slides
It may lower low quality images
Dry mount slide
There is heat involved
Heat Fixed Slide
Immersion oil is placed directly on the slide and the 100x oil immersion objective is used to observe the bacteria
Heat Fixed Microscope Slide
Smears, its angle determines the length of the smear; a steeper angle corrects a shorter smear
Smear Slide
Designed for soft samples, literally squishes the specimen
Squash slides
Stains the slides, samples such us iodine, methylene blue, and crystal violent
Staining slides
Use for cutting sections at a specific thickness by sliding a block i to a cutting tool
Microtome
Where the tissue is held in position
Block holder
For the actual cutting and experience
Knife and carrier and knife
To line up the tissue block in the proper position with the knife
Pawl, Ratchet feed wheel and adjustment screws
Cutting serial sections of large blocks of paraffin embedded tissues
Rocking Microtome
For cutting paraffin embedded section
Rotary Microtome
For cutting celloidin embedded section
Sliding Microtome
For cutting unembedded frozen sections
Freezing Microtome
For cutting frozen sections
Cryostat or Cold Microtome
For cutting sections for electron microscopy
Ultrathin Microscope