Tubes Additives Order Of Draw Flashcards
Product of centrifuged blood
- Cellular elements
- Supernatant, light yellow tinted fluid
Plasma
Liquid portion of unclotted blood and still contain the clotting factors
Serum
Liquid portion of blood that has been allowed to clot(coagulate)
Clotting factors are no longer present
Found in tubes without anticoagulant
Which tubes are with anticoagulant and produce plasma when centrifuged?
Lavender
Light green
Dark green
Pink
Light blue
Royal blue with lavender stripe
Gray
Which tubes are without anticoagulants and hence produce serum when centrifuged?
Clear(used as discard tube)
Red-glass
Red-plastic (will have activator)
Tiger too/Gold top/Marble top
Royal blue with a red stripe
Collecting specimens in the incorrect order of draw can result?
Cross contamination
Who has defined the order of draw to minimize cross contamination?
The clinical and laboratory standards Institeut(CLSI)
The CLSI approved order of draw is?
Blood culture bottle
Discard tube(clear or red)
Light blue top
Red top
Gold/Tiger/Marble Top
Light green top
Dark green top
Pink top
Levander top
Gray top
*Dark blue (as called for I draw order)
Blood culture bottle additives
SPS(sodium poly ethanol sulfate) or nutrient broth
The most common cause of blood culture contamination is
Improper site preparation
-venipuncture site is cleansed with
-the top of the collection bottle should be cleaned
What are the discard tubes?
Clear top or Red top tubes
What is the the function, additive function and order of Light blue top tube.
Additive: Sodium citrate- anticoagulant
Yield: plasma
Purpose: coagulation or hematology
Draw clear top or red top tube before drawing light blue top tube( to prevent thromboplastine )
Blood to additive ratio in light blue top tube
The ratio is 9 parts blood to 1 part additive ( sodium citrate)
There is a line mark on the tube to insure the proper amount
Invert 3-4 times
What can affect coagulation study results related to sample collection?
Both thromboplastine and air can affect the coagulation study results
PT
Extrinsic system
Monitoring Coumadin
PTT or aPTT
Test the same function
In aPTT activator is added, evaluates how long the patients blood takes to form a clot
Evaluate intrinsic system of the coagulation cascade
Monitoring patients on heparin
Other coagulation test done with light blue top tube
D-dimmer test
Light blue topped tubes are also used to prepare what blood components therapy
Platlate rich plasma
What is the additive, yield and department responsible for Red top tube?
Additive is none for glass and clot activator for plastic
Yield is serum
Department responsible is chemistry
Blood will naturally clot within 30 to 60 minutes
Serum pregnancy test and Alcohol level can be tested. It is sometimes used to to obtain a purer sample.
What are the other names for serum separation tube?
Tiger top tube, marble top tube red gray specked top tube, Gold top tube or Gel tube
What are the additives and the yield of SST? Which department does it belong?
Silica(clot activator) and Thioxotropic Gel(serum separator)
It yields serum and
Belongs to the chemistry department
What tests are performed using the Tiger Top tubes
Complete and basic metabolic panels
Serum pregnancy test
Lipid and cholesterol
C- reactive protein test
What is the other name for light green top tube?
Lithium tope tube
What is the other name for light green top tube?
Plasma separation tube
What are the additives of light green top tube?
Thixotropie Gel and Heparne
How many times should the light(lithium Heparin) top tube must be inverted
8 times
Common tests done with light green top tube
State electrolytes
Plasma hCG(both qualitative and quantitative)
Light green top tube
Lithium heparin
Plasma
Chemistry
Dark Green top tube
Additive is sodium heparin
Yield is plasma
Lab responsible is chemistry
Must be inverted 8 times
Common test is ammonia
Doesn’t have Jel additive
*ammonia must be placed on ice
Pink top tube
Additive is potassium EDTA
YIELD is plasma
Laboratory responsible is Blood Bank
The blood sample must feel the tube to 2/3 or more
Must be turned 8 times
Used for molecular diagnostic and viral loads
Common tests done are type and screen ratio and cross matches
Lavender or light/dark purple top tube
Additive is EDTA( inhibit coagulation by binding to calcium)
Yield is plasma
Laboratory responsible is Hematology
Common tests
-CBC
-ESR
-Hct
-Hb
-platelets
-C-reactive proteins
*CBC should never be centrifuged
Must be filled 2/3 and turned 8 times
Gray top tube
Additives are sodium fluoride(antiglycolytic agent/ glucose preservative, can preserve glucose for 3days) and potassium oxalate(anticoagulant)
Yield is plasma
Lab responsible is chemistry
Common tests are OGTT , lactic acid and alcohol levels
* lactic acid should be drawn with out tourniquet and placed in ice
*when drawing for alcohol level do not clean the site with alcohol
Royal blue top tube
Additive is non is none for red stripe and EDTA foe blue/lavender stripe
Yield can be serum or plasma
Lab is Toxicology
Common tests are lead and heavy metal toxicology
CLSI order of draw chart
Blood culture bottle
Or
Red top
Light blue top
Royal blue with red stripe
Gold/Tiger/Marble top
Light free top
Dark green top
Pink top
Levander top
Royal blue with levander stripe
Gray top
