Tubes, Additives and Tests

Question 1

Lavender top tube contains

Answer 1

EDTA aka Ethylenediamine tetraacetic acid

Question 2

EDTA function

Answer 2

inhibits coagulation by binding to calcium present in the specimen.

Question 3

How much should the lavender tube be filled?

Answer 3

The tube must be filled at least 2/3 full

Question 4

How many times should you invert the lavender tube?

Answer 4

8 times

Question 5

What are the common tests for lavender top tubes?

Answer 5

Hematology tests such as:
CBC (Complete Blood Count); Includes: RBC count, WBC count and Platelet count; WBC differential count; Hemoglobin and Hematocrit
determinations; ESR (Erythrocyte Sedimentation Rate); Sickle Cell Screening

Question 6

Lavender top tubes Indicated to test for

Answer 6

whole blood

Question 7

Light-Blue top tube contains

Answer 7

Contains the anticoagulant Sodium Citrate

Question 8

Tubes used for anticoagulant blood tests

Answer 8

tubes used are those with light blue, green, gray, dark blue, and lavender or pink stoppers.

Question 9

Sodium Citrate function

Answer 9

prevents coagulation by binding to calcium in the specimen.

Question 10

Light Blue top tube used for

Answer 10

coagulation studies because it preserves the coagulation factors

Question 11

How much should you fill the light blue top tube?

Answer 11

The tube must be filled completely to maintain the ratio of nine parts blood to one part sodium citrate

Question 12

How many times should the light blue top tube be inverted?

Answer 12

should be inverted three to four times..

Question 13

What are common tests for the light blue top tubes?

Answer 13

Coagulation Studies- Prothrombin Time (PT) – evaluates the extrinsic; system of the coagulation cascade and monitors; Coumadin therapy; Activated Partial Thromboplastin Time (APTT, PTT) - Evaluates the intrinsic system of the coagulation cascade and monitors Heparin therapy. Fibrinogen Degradation Products (FDP) Thrombin Time (TT); Factor assays, Bleeding Time (BT)

Question 14

Green top tube contains

Answer 14

Contains the anticoagulant Heparin combined with sodium, lithium, or ammonium ion.

Question 15

Function of Heparin

Answer 15

Anticoagulant that works by inhibiting thrombin in the coagulation cascade

Question 16

Common tests for the green top tube

Answer 16

It is not used for hematology because heparin interferes with the Wright‟s stained blood smear.

Chemistry tests: performed on plasma such as Ammonia, carboxyhemoglobin & STAT electrolytes

Question 17

Common tests for the green top tube

Answer 17

It is not used for hematology because heparin interferes with the Wright‟s stained blood smear.

Chemistry tests: performed on plasma such as Ammonia, carboxyhemoglobin & STAT electrolytes

Question 18

Gray top tube contains

Answer 18

Contains additives and anticoagulants. All gray top tubes contain glucose preservative (antiglycolytic agent): sodium fluoride- preserves glucose for 3days; or lithium iodoacetate- preserves glucose for 24 hours. May also contain the anticoagulant potassium oxalate, which prevents clotting by binding calcium.

Question 19

Common tests for the gray top tube

Answer 19

Fasting blood sugar (FBS); Glucose tolerance test (GTT); Blood alcohol levels; Lactic acid measurement

Question 20

Red/Gray (speckled) top tube known as

Answer 20

tiger-top tube and serum separator tubes (SST).

Question 21

Red/Gray (speckled) top tube contains

Answer 21

Contain clot activators:
glass particles, silica and celite which hastens clot formation, and thixotropic gel, a serum separator which when centrifuged forms a barrier between the serum and the cells preventing contamination of the serum with cellular elements

Question 22

How many times should you invert tiger top tube?

Answer 22

Tubes must be inverted five times.

Question 23

Common tests of the Red/Gray (speckled) top tube

Answer 23

Most chemistry tests

Question 24

Which tube should be inverted 3-4 times?

Answer 24

Light blue topped tube

Question 25

Which tube should be inverted 5 times?

Answer 25

Tiger topped or gold topped tube

Question 26

A Phlebotomist has a requisition to draw blood on a patient who has severe Downs yndrome. which of the following steps should the phlebotomist take?

Answer 26

Speak with the patient’s guardian before preforming the venipuncture to ensure the explanation is understood.

Question 27

which of the following is an example of the acceptable reporting of routine lab results?

Answer 27

send the results to the physician in a marked envelope.

Question 28

Which of the following procedural steps for a finger stick should the phlebotomist do first?

Answer 28

confirm the correct identity of the patient

Question 29

A phlebotomist is drawing blood from a patient and observes a red spot. Which of the following is an appropriate action for the phlebotomist to take?

Answer 29

Apply pressure after collecting the specimen.

Question 30

Which of the following is appropriate to use when collecting for a chemistry test that requires serum?

Answer 30

SST

Question 31

A phlebotomist working in a fertility clinic draws blood from a patient who also must
submit a semen sample for analysis. Which of the following should the phlebotomist
tell the patient?

Answer 31

“As soon as you can collect the specimen, keep it next to your body. We will need to submit it right away for testing.”

Question 32

Which of the following test results are affected when providone iodine is used to cleanse the state for a dermal puncture?

Answer 32

Potassium