TSH, T3, T4

Question 1

Principle of TSH ELISA.

Answer 1

The TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay.

Question 2

The assay system utilizes a _______________ directed against a ________________ on the _____________.

Answer 2

unique monoclonal antibody, distinct antigenic determinant, intact TSH molecule

Question 3

______________ antibody is used for ______________ (on the microtiter wells). A _______________ is in the antibody-enzyme conjugate solution.

Answer 3

Mouse monoclonal anti-TSH, solid phase immobilization, goat anti-TSH antibody

Question 4

What enzyme is in the goat anti-TSH?

Answer 4

horseradish peroxidase

Question 5

The test sample is allowed to react simultaneously with the two antibodies, resulting in the TSH molecules being sandwiched between the solid phase
and enzyme-linked antibodies. The wells are washed to remove _______________.

Answer 5

unbound labeled antibodies

Question 6

A solution of __________ is added for _____________. The color development is stopped with the addition of Stop solution, changing the color to ________.

Answer 6

TMB reagent, blue color development, yellow

Question 7

The concentration of _____ is directly proportional to the ________ of the test sample. Absorbance is measured spectrophotometrically at _______.

Answer 7

TSH, color intensity, 450nm

Question 8

The ________ exerts powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance, as well as on the maintenance of metabolic activity and development of skeletal and organ system.

Answer 8

thyroid gland

Question 9

The hormones _______________ circulate in the bloodstream, mostly bound to the
plasma protein, ____________.

Answer 9

thyroxine (T4) and 3,5,3’-triiodothyronine (T3), thyroxine binding globulin (TBG)

Question 10

its metabolic potency is much greater.

Answer 10

T3

Question 11

___ determination is an important factor in the diagnosis of _______. Its measurement has uncovered a variant of ___________ with elevated T3 levels and normal T4 levels.

Answer 11

T3, thyroid disease, hyperthyroidism in thyrotoxic patients

Question 12

It is useful in monitoring patients under treatment for hyperthyroidism and those who have discontinued anti-thyroid drug therapy. It is especially valuable in distinguishing between ____________. In women, T3 levels are elevated during ________, during _____________________.

Answer 12

euthyroid and hyperthyroid subject, pregnancy estrogen treatment and contraceptive hormone therapy