troubleshooting Flashcards
1
Q
increased band intensity
A
decreased width of well
1
Q
poor resolution
A
wrong concentration; comb too narrow
2
Q
fuzzy bands
A
diffusion of DNA through gel
3
Q
band distortion
A
dried agarose on comb, incomplete melting of agarose
4
Q
smearing
A
overloading, overheating, sample well torn
5
Q
melted gel during run
A
heat, high voltage, high ionic strength
6
Q
DNA did not move out of well
A
incorrect set up of box, no power connection
7
Q
bands smiling across entire gel
A
uneven dissipation of heat through gel, sides cooler than center, re-circulate buffer during run
8
Q
band smiling/ frowning within lane
A
salt impeding migration, salt/solvent issues in sample
9
Q
A
