TREMATODES Flashcards
TREMATODES IS ALSO KNOWN AS
FLUKES
WHAT IS THE SHAPE OF TREMATODES?
LEAF-LIKE (ADULT)
TREMATODES IS A _________ / __________, WHICH MEANS IT HAS A MALEA ND FEMALE REPRODUCTIVE ORGANS
MONOECIOUS / HERMAPHRODITIC
THE INFECTIVE STAGE OF TREMATODES
METACERCARIA
THE LAST LARVAL STAGE OF TREMATODES
METACERCARIA
ATTACH THEMSELVES TO THE HOST BY MEANS OF 2 SUCKERS
TREMATODES
TREMATODES ATTACH THEMSELVES TO THE HOST BE MEANS OF _______
2 SUCKERS
PARTS OF TREMATODE SUCKERS
- ORAL SUCKER
- VENTRAL SUCKER / ACETABULUM
3RD SUCKER SEEN IN HETEROPHYES HETEROPHYES
GENITAL SUCKER / GONOTYL
THE 3RD SUCKER (GENITAL SUCKER / GONOTYL) IS SEEN IN
HETEROPHYES HETEROPHYES
TREMATODES:
1ST INTERMEDIATE HOST
SNAILS
TREMATODES:
2ND INTERMEDIATE HOST
FISH, CRABS, VEGETATION, SNAIL
TREMATODES:
MODE OF TRANSMISSION
INGESTION of 2nd intermediate host infected w/
metacercaria
TREMATODES:
Has operculum, lid-like structure allowing the exit of
larva when they hatch
EGGS
Eggs w/ operculum are called
OPERCULATED OVA
TREMATODES:
LARVAL STAGES
- MIRACIDIUM
- SPOROCYST
- REDIA
- CERCARIA
- METACERCARIA
Tips (Mnemonic): My Sister Rent a Car for Me
ADULT OVA
Oviparous or oviviparous
Trematodes resides in multiple areas of the body:
Liver
Intestine
Pancreas
Lungs
GIVE THE LIVER FLUKES
- FASCIOLA HEPATICA
- FASCIOLA GIGANTICA
- CLONORCHIS IMMITIS
- OPISTHORCHIS FELINEUS
- DICROCOELIUM LANCEOLATA
GIVE THE LIVER FASCIOLIDS
- FASCIOLA HEPATICA
- FISCIOLA GIGANTICA
FASCIOLA HEPATICA IS ALSO KNOWN AS
SHEEP LIVER FLUKE
TEMPERATE LIVER FLUKE
FASCIOLA GIGANTICA IS ALSO KNOWN AS
GIANT LVIER FLUKE
TROPICAL LIVER FLUKE
THE LARGEST LIVER FLUKE
FASCIOLA GIGANTICA
are termed to be Fasciolids
Fasciola hepatica & Fasciola gigantica
NATURAL / FINAL HOST OF Fasciola hepatica
SHEEP
NATURAL / FINAL HOST OF FASCIOLA GIGANTICA
- LOCAL CATTLE
- HERBIVORES
serves as an accidental host for Fasciola hepatica & Fasciola gigantica
man
(when man accidentally ingest a 2nd intermediate host infected with metacercaria of either F. hepatica or F. gigantica, they may acquire liver fluke)
1st Intermediate Host of Fasciola hepatica & Fasciola gigantica
snail
- Lymnea philippinensis
- Lymnea auricularia rubiginosa
2nd Intermediate Host of Fasciola hepatica & Fasciola gigantica
vegetations
- Ipomea obscura (kangkong)
- Nasturtium officinale (watercress)
habitat for Fasciola hepatica & Fasciola gigantica
liver parenchyma and gallbladder
infective stage for Fasciola hepatica & Fasciola gigantica
metacercaria
pathology for Fasciola hepatica & Fasciola gigantica
- fascioliasis (sheep liver rot / liver rot)
- liver atrophy
- pharyngeal suffocation
it is the result of the temporary lodgement of the fluke in the pharynx. give the MOT.
pharyngeal suffocation
ingestion
give 2 terms of pharyngeal suffocation
halzoun and mararra
pharyngeal suffocation terms:
originated from Lebanon
halzoun
pharyngeal suffocation terms:
originated from Sudan
mararra
laboratory diagnosis for Fasciola hepatica & Fasciola gigantica
stool exam
liver biopsy
PCR
drug of choice for Fasciola hepatica & Fasciola gigantica
Bithionol
Triclabendazole
LIVER FASCIOLIDS (MORPHOLOGY OF ADULT)
with cephalic cone which has well developed shoulder
Fasciola hepatica
LIVER FASCIOLIDS (MORPHOLOGY OF ADULT)
larger than F. hepatica
Fasciola gigantica
LIVER FASCIOLIDS (MORPHOLOGY OF ADULT)
less developed shoulder
Fasciola gigantica
LIVER FASCIOLIDS (MORPHOLOGY OF ADULT)
shorter cephalic cone
Fasciola gigantica
MORPHOLOGY OF ADULT (FASCIOLA HEPATICA)
with cephalic cone which has well developed shoulder
MORPHOLOGY OF ADULT (FASCIOLA HEPATICA)
- larger than F. hepatica
- less developed shoulder
- shorter cephalic cone
MORPHOLOGY OF LARVA (LIVER FASCIOLOIDS)
ALL STAGES ARE PRESENT
Miracidium
Sporocyst
Redia
Cercaria
Metacercaria
MORPHOLOGY OF EGG (LIVER FASCIOLOIDS)
large, operculated, unembryonated, yellowish-brown egg (Hen’s egg shape)
are termed to be Heterophyids
Clonorchis sinensis & Opisthorchis felineus
most important liver fluke of man
Clonorchis sinensis
a potential carcinogen and may cause liver dysfunction
Clonorchis sinensis
first described by McConnell
Opisthorchis felineus
other specie of opistorchis
Opistorchis viverrini
Opistorchis viverrini is also known as
Southeast Asian Liver Fluke
CLONORCHIS SINENSIS other names
Oriental Liver Fluke
Chinese Liver Fluke
OPISTORCHIS FELINEUS other names
Cat Liver Fluke
Siberia Liver Fluke
final host of clonorchis sinensis
man and other fish-eating vertebrae
final host of opisthorchis felineus
cats (cat liver fluke)
1st intermediate host of clonorchis sinensis
SNAIL
Family of the ff.:
Parafossarulus
Bulinus
Semisulcospira
Alocinma
Thiara
Melanoide
1st intermediate host of opisthorchis fenineus
SNAIL
Family Bithynia
2nd intermediate host of clonorchis sinensis
FISHES
Family Cyprinidae
2nd intermediate host of opisthorchis felineus
FISHES
Family of the ff.:
Cyprinidae
Cobitidae
HABITAT FOR BOTH CLINOCHIS SINENSIS AND OPISTHORCHIS FELINEUS
- LIVER PARENCHYMA
- ALSO IN PANCREATIC DUCT AND GALLBLADDER
INFECTIVE STAGE FOR CLONORCHIS SINENSIS AND OPISTHORCHIS FELINEUS
METACERCARIA
PATHOLOGY FOR CLONORCHIS SINENSIS AND OPISTHORCHIS FELINEUS
- periductal fibrosis
- gallbladder and liver carcinoma causing:
~ hepatocellular carcinoma
~ cholangiocarcinoma
~ most likely caused by C. sinensis - liver dysfunction
~ caused by C. sinensis
~ occurs on person severely infected over a long period of time - bile duct obstruction
LABORTORY DIAGNOSIS FOR CLINORCHIS SINENSIS AND OPISTHORCHIS FELINEUS
- stool exam
- serologic tests
- molecular tests
DRUG OF CHOICE FOR CLONORCHIS SINENSIS AND OPISTHORCHIS FELINEUS
PRAZIQUANTEL AND ALBENDAZOLE
MORPHOLOGY OF ADULT (CLONORCHIS SINENSIS AND OPISTHORCHIS FELINEUS)
- leaf-like with transparent tegument
- has vitellaria found in the middle third of the body
gravid uterus; uterus filled with ova
vitellaria
two large, highly branched testes arranged in tandem
Clonorchis sinensis (ADULT MORPHOLOGY)
lobate testes arranged obliquely
Opisthorchis felineus (ADULT MORPHOLOGY)
Opisthorchis felineus (SPECIFIC ADULT MORPHOLOGY)
lobate testes arranged obliquely
Clonorchis sinensis (SPECIFIC ADULT MORPHOLOGY)
two large, highly branched testes arranged in tandem
Morphology of Adult
for both:
- _________ with transparent tegument
- has _________ found in the middle third of the body
~ __________ - gravid uterus; uterus filled with ____
Clonorchis sinensis (SPECIFIC)
- two large, highly branched testes arranged in _________
Opisthorchis felineus (SPECIFIC)
- _________________ arranged obliquely
Morphology of Adult
for both:
- leaf-like with transparent tegument
- has vitellaria found in the middle third of the body
~ vitellaria - gravid uterus; uterus filled with ova
Clonorchis sinensis (SPECIFIC)
- two large, highly branched testes arranged in tandem
Opisthorchis felineus (SPECIFIC)
- lobate testes arranged obliquely
Morphology of Egg/Ova (for C. SINESIS AND O. FELINEUS):
- ________________, ovoid (pitcher-shaped / old-fashioned electric light bulb-shaped)
- distinct ___________________ that fits to the thickened rim of the eggshell
- thick rim located around the operculum (shoulders)
- small protuberance at the ____________________
~ in the opposite pole of the operculum
~ usually a ________-shaped
Morphology of Egg/Ova (for C. SINESIS AND O. FELINEUS):
- yellowish-brown, ovoid (pitcher-shaped / old-fashioned electric light bulb-shaped)
- distinct convex operculum that fits to the thickened rim of the eggshell
- thick rim located around the operculum (shoulders)
- small protuberance at the abopercular end
~ in the opposite pole of the operculum
~ usually a comma-shaped
SHAPE OF THE EGG/OVA OF C. SINENSIS AND O. FELINEUS
pitcher-shaped / old-fashioned electric light bulb-shaped
TRUE OR FALSE:
The egg/ova of fasciolids and heterophyids are distinguishable
FALSE
The egg/ova of fasciolids and heterophyids are reported as
fasciolid ova or heterophyid ova
responsible for human infection in West Africa
Dicrocoelium hospes
Dicrocoelium dendriticum AKA
Lanceolate / Lancet Fluke
final host of Dicrocoelium dendriticum
cattle / sheep
**also man serves as a final host
intermediate host of Dicrocoelium dendriticum
SNAIL
- Cochlicopa
- Helix
- Xerolenta
- Zebrina
2ND INTERMEDIATE HOST OF Dicrocoelium dendriticum
ants (Formica fusca)
- black ants
HABITAT OF Dicrocoelium dendriticum
biliary passage of the liver
infective stage of Dicrocoelium dendriticum
metacercaria
MOT of Dicrocoelium dendriticum
ingestion of 2nd intermediate host with metacercaria
pathology of Dicrocoelium dendriticum
cirrhosis / fibrosis of the liver
laboratory diagnosis of Dicrocoelium dendriticum
liver biopsy and PCR
morphology of adult Dicrocoelium dendriticum
- _______ and ________ at both sides
- _______-shaped
- flattened and tapered at both sides
- lancet-shaped
morphology of egg/ova Dicrocoelium dendriticum
- thick-shelled, operculated, and _________________
thick-shelled, operculated, and deep golden brown
INTESTINAL FLUKES
Fasciolopsis buski
Echinostoma ilocanum
Heterophyes heterophyes
Metagonimus yokogawai
3rd specie under the fasciolids
Fasciolopsis buski
largest fluke parasitizing man
Fasciolopsis buski
larger than F. gigantica
Fasciolopsis buski
Fasciolopsis buski aka
Giant Intestinal Fluke
final host of Fasciolopsis buski
man
1st intermediate host of Fasciolopsis buski
SNAIL
- segmentina
- hippeutis
2nd intermediate host of Fasciolopsis buski
VEGETATION
- Water caltrop (Trapa bicornis)
- Water chestnut (Eliocharis tuberosa)
- Kangkong / Morning Glory (Ipomea obscura)
- Lotus (Nymphoea lotus)
- Bamboo shoots (Zizania latifolia)
HABITAT of Fasciolopsis buski
small intestine
infective stage of Fasciolopsis buski
metacercaria
pathology of Fasciolopsis buski
- abdominal discomfort
- diarrhea
- edema
laboratory diagnosis of Fasciolopsis buski
- stool exam
- concentration techniques
- molecular techniques
drug of choice of Fasciolopsis buski
Praziquantel
Morphology of Adult (Fasciolopsis buski)
- has simple intestinal ceca
- two dendritic testes in tandem
- branched ovaries
Morphology of Egg (Fasciolopsis buski)
indistinguishable from other fasciolid eggs
discovered by Philip Garrison among prisoners of Bilibid Prison in Manila, PH
Echinostoma ilocanum
Echinostoma ilocanum aka
Garrison’s Fluke
final host of Echinostoma ilocanum
man
1st intermediate host Echinostoma ilocanum
SNAIL
- Gyraulus convexiusculus
- Hippeutis umbilicalis
2ND INTERMEDIATE HOST OF Echinostoma ilocanum
SNAIL
- Kuhol (Pila luzonica)
- Susong pampang (Vivipara angularis)
HABITAT OF Echinostoma ilocanum
SMALL INTESTINE
infective stage of Echinostoma ilocanum
metacercaria
pathology of Echinostoma ilocanum
- abdominal discomfort
- chronic mucous diarrhea
- eosinophilia
laboratory diagnosis of Echinostoma ilocanum
-stool exam
- concentration techniques
- molecular techniques
drug of choice of Echinostoma ilocanum
Praziquantel
morphology of adult (Echinostoma ilocanum)
circumoral disk w/ _____________ (_____________) surrounding oral sucker
circumoral disk w/ crown of spines (49-51 spines) surrounding oral sucker
morphology of egg (Echinostoma ilocanum)
germ ball egg w/ ___________ operculum
germ ball egg w/ triangular operculum
smallest trematode of man
Heterophyes heterophyes
deadliest trematode;
as it can migrate to:
Heterophyes heterophyes
heart, brain, spinal cord
causes Heterophyid myocarditis; 15% of total fatal heart disease in PH
Heterophyes heterophyes
Heterophyes heterophyes aka
Von Siebold’s Fluke
Heterophyid Fluke
final host of Heterophyes heterophyes
man
1st intermediate host of Heterophyes heterophyes
SNAIL
- Cerithidea
- Pirenella
2nd intermediate host of Heterophyes heterophyes
FISHES
- Balanak (Mugil spp.)
- Tilapia (Tilapia spp.)
- Kanduli (Arius manilensis)
habitat of Heterophyes heterophyes
SMALL INTESTINE
infective stage of Heterophyes heterophyes
METACERCARIA
pathology of Heterophyes heterophyes
- abdominal pain
- mucous diarrhea
- ulceration of intestinal wall
laboratory diagnosis of Heterophyes heterophyes
- stool exam
- concentration techniques
- molecular techniques
drug of choice of Heterophyes heterophyes
Praziquantel
morphology of adult (Heterophyes heterophyes)
has genital sucker / gonocyte / gonotyl (3rd sucker) surrounding the genital pore
morphology of egg (Heterophyes heterophyes)
- old-fashioned light bulb
- same as heterophyids except for the presence of small protuberance at abopercular end
included in the heterophyids
Metagonimus yokogawai
Metagonimus yokogawai aka
yokogawa’s fluke
final host of Metagonimus yokogawai
man
1st intermediate host of Metagonimus yokogawai
SNAIL
- Semisulcospira spp.
2nd intermediate host of Metagonimus yokogawai
FISHES
- Salmonoid fishes
- Cyprinoid fishes
habitat of Metagonimus yokogawai
small intestine
infective stage of Metagonimus yokogawai
metacercaria
MOT of Metagonimus yokogawai
Ingestion of 2nd Intermediate Host with Metacercaria
morphology of adult (Metagonimus yokogawai)
pyriform-shaped scaly spines, no gonotyl
morphology of egg (Metagonimus yokogawai)
- similar with Heterophyes spp.
- diff. to others: has thin shell
LUNG FLUKE
Paragonimus westermani
common in Sorsogon
Paragonimus westermani
Paragonimus westermani aka
Oriental Lung Fluke
final host of Paragonimus westermani
man
1st intermediate host of Paragonimus westermani
SNAIL
- Antemelania asperata (formerly Brotia asperata)
- Antemelania dactylus
2nd intermediate host of Paragonimus westermani
CRABS
Sundathelphusa philippina (formerly Parathelpusa grapsoides)
habitat of Paragonimus westermani
LUNGS
infective stage of Paragonimus westermani
METACERCARIA
MOT of Paragonimus westermani
ingestion of the 2nd intermediate host with Metacercaria
pathology of Paragonimus westermani
Paragonimiasis
causes pulmonary distomiasis
Paragonimiasis
chronic cough and hemoptysis (coughing with blood
Paragonimiasis
resembles tuberculosis
Paragonimiasis
morphology of adult (Paragonimus westermani)
- coffee-bean shaped
- wavy or succulent intestinal ceca
morphology of egg (Paragonimus westermani)
- thickened abopercular end
- flattened but prominent operculum
- similar w/ Diphyllobothrium latum (a cestode)
EGG IS similar w/ Diphyllobothrium latum
Paragonimus westermani
LABORATORY DIAGNOSIS OF Paragonimus westermani
- sputum examination
~ ova + Charcot-Leyden crystals
- may also look for adult
- eosinophil degradation crystal - stool exam (DFS)
sputum may be swallowed, transferring ova to stool - chest x-ray
- serodiagnostics (ELISA, IB)
- molecular tests (PCR)
AKA pancreatic fluke
Eurytrema pancreaticum
Final host of Eurytrema pancreaticum
cattle/sheep
1st intermediate host Eurytrema pancreaticum
Snail
2nd intermediate host Eurytrema pancreaticum
Ants
Grasshoppers
Mantis
Crickets
Habitat of Eurytrema pancreaticum
Pancreatic ducts
Infective stage Eurytrema pancreaticum
Metacercaria
MOT of Eurytrema pancreaticum
Ingestion of 2nd IH with metacercaria
Adult Eurytrema pancreaticum
Ruffled margins
Egg Eurytrema pancreaticum
Operculated, embryonated
Blood flukes aka
Schistosomes
Separate sexes; adult male/adult female
dioecious
Blood flukes shape
Elongated/cylindrical
Intermediate host of Eurytrema pancreaticum
Snail
Infective stage of Eurytrema pancreaticum
Miracidia - snail
Cercaria - final host
MOT of Eurytrema pancreaticum
Skin penetration in adult
Habitat of Eurytrema pancreaticum
Blood vessels
Adult Eurytrema pancreaticum is called as
Perpetual copulation
Adult Eurytrema pancreaticum is known as
Most romantic parasite
Female adult of Eurytrema pancreaticum
Oviviparous
Male adult of Eurytrema pancreaticum
Have gynecophoral canal/grooves
This part holds the female
Gynecophoral canal
T/F
Female Eurytrema pancreaticum are larger than male
False
Common in mindoro, leyte,sorsogon, mindanao
Schistosoma japonicum
Schistosoma japonicum aka
Oriental blood fluke
Schistosoma japonicum is the ______ ova among blood flukes
Smallest
Schistosoma japonicum is the ____ adult among blood flukes
Largest
Intermediate host of Schistosoma japonicum
Oncomelania hupensis quadrasi (snail)
Final host of Schistosoma japonicum
Man
Habitat of Schistosoma japonicum
Superior mesenteric vein
Infective stage of Schistosoma japonicum
Cercaria (forked tail)
MOT of Schistosoma japonicum
Skin penetration
No tail/tailless Cercaria
schistosomules/schistosomula (when the Cercaria penetrates the skin of the host, they lose their tail)
Disease related to Schistosoma japonicum
Katayama Disease (snail fever)
Oriental Schistosomiasis (swamp fever)
First reported by Theofore Bilharz
Schistosoma mansoni
Laboratory diagnosis for Schistosoma japonicum
stool exam
rectal biopsy specimen
Adult morphology of Schistosoma japonicum
Smooth integument
Morphology of egg Schistosoma japonicum
knob-like or curved hook of protuberance on one side (lateral knob)
Common name for Schistosoma mansoni
Mansoni blood fluke
Final host of Schistosoma mansoni
Man
Intermediate host of Schistosoma mansoni
Snail
Biomphalaria spp.
Australorbis spp.
Tropicorbis spp.
Planorbis spp.
Habitat of Schistosoma mansoni
Inferior mesenteric vein of colon/rectum
Infective stage of Schistosoma mansoni
Cercaria (forked tail)
MOT of Schistosoma mansoni
Skin penetration
Pathology of Schistosoma mansoni
Intestinal Bilharziasis
Laboratory diagnosis of Schistosoma mansoni
stool exam
rectal biopsy specimen
Smallest blood fluke
Schistosoma mansoni
Egg Morphology of Schistosoma mansoni
Light yellowish brown
Appearance of egg Schistosoma mansoni
Rose-thorn
Common in Nile River Valley
Schistosoma haematobium
Common name of Schistosoma haematobium
Vesical blood fluke
Final host of Schistosoma haematobium
Man
Intermediate host of Schistosoma haematobium
Snail
Bulinus spp.
Physopsis spp.
Biomphalaria spp.
Habitat of Schistosoma haematobium
Vesical, prostatic, uterine venous plexuses of urinary bladder
Infective stage of Schistosoma haematobium
Cercaria (forked tail)
MOT of Schistosoma haematobium
Skin penetration
Disease related to Schistosoma haematobium
Urinary Bilharziasis
Laboratory diagnosis for Schistosoma haematobium
Concentrated urine spx
Preferred specimen for Schistosoma haematobium
24-hr unpreserved urine
Preservation of urine might result to
Destruction of ova
Optimized recovery in urine
Between noon and 2 pm
Morphology of adult Schistosoma haematobium
Fine tuberculation
Egg morphology of Schistosoma haematobium
Large, prominent terminal spine
Dermatitis with pruritus at site of cercarial penetratIon
Swimmer’s Itch / Gulf-Coast Itch / Cercarial Itch / Clam Digger’s Itch
systemic hypersensitivity reaction to schistosomulum migrating thru tissue
Katayama Disease / Katayama Fever / Snail Fever
Katayama Disease / Katayama Fever / Snail Fever is caused by
Schistosoma japonicum
most serious consequence of chronic schistosomiasis
Hepatosplenic Schistosomiasis
infected patients have enlarged stomachs due to large amounts of peritoneal fluid
Ascites
Characterized by hematuria
Urinary Bilharziasis / Egyptian Hematuria
Urinary Bilharziasis / Egyptian Hematuria presents ___ in lower urinary tract
Sandy patches
Urinary Bilharziasis / Egyptian Hematuria is caused by
Schistosoma haematobium
Both are found in mesenteric vein
S. japonicum and S. mansoni
Laboratory diagnosis for S. japonicum and S. mansoni
Stool Exam
Rectal Biopsy
Sedimentation Technique
faust-meleney’s egg hatching technique
Circumoval Precipitin Test (COPT)
Cercarial Hullen Reaction
Miracidial egg hatching technique
faust-meleney’s egg hatching technique
Water used in faust-meleney’s egg hatching technique
Non-chlorinated water
Specimen used in faust-meleney’s egg hatching technique
freshly collected stool or urine (S. haematobium)
Process of freshly collected stool or urine (S. haematobium)
dissolve sample in non-chlorinated water placed in erlenmeyer flask
cover and stand overnight against light
incubate and observe for miracidia swimming at upper layer of water
Definitive test for schistosomiasis in PH
Circumoval Precipitin Test (COPT)
Specimen used in Circumoval Precipitin Test (COPT)
Serum
Reagent used in Circumoval Precipitin Test (COPT)
Lyophilized eggs from rabbit
Circumoval Precipitin Test (COPT) demonstrates ______, microscopically
bleb formation
Specimen used cercarial hullen reaction
Serum
Reagent used in cercarial hullen reaction
lyophilized cercaria
cercarial hullen reaction demonstrates
Shrinking of cercaria
Discovered in mekong river
Schistosoma mekongi
Adult Schistosoma mekongi reside in
Small intestine venules
Egg of Schistosoma mekongi resembles what
Schistosoma japonicum (but smaller)
Adult Schistosoma intercalatum reside in
colon/large intestine venules
Egg of Schistosoma intercalatum resembles what
egg of S. haematobium (with equatorial/central bulge