transplant Flashcards
autotransplantation
transplant tissue from one part of the body to another
allotransplantation
transplant tissue from one person to another
orthotopic transplant
transplanted organ is placed in the same location at the original organ (heart, lung, liver)
heterotopic transplant
transplanted organ is placed in a different location as the original organ (pancreas, kidney)
which organs can have deceased or living donors
kidney, liver
which organs can have deceased donors only
heart, lung, pancreas
how does the immune system identify “self”
a self marker (MHC) labels the body’s cells as a friend
how does the immune system identify “non-self”
it recognizes an antigen as foreign and treats it as a foe
how do HLA molecules act in transplant
they are markers that determine compatibility of tissue for transplantation
MHC class I
found on all nucleated cells. presents antigenic peptides to CD8+ T cells
MHC class II
restricted expression on antigen presenting cells
presents antigenic peptides to CD4+ T cells
Type A blood
can only have A or O blood
Type B blood
can only have B or O blood
Type AB blood
universal recipient
Type O blood
universal donor
how do patients develop antibodies to other HLAs
transfusion, pregnancy, other organ transplant
sensitization is generally defined as PRA > __%
10
recipient risk factors for rejection
black race, highly sensitized, previous transplant, young age, prior pregnancy
when does hyperacute rejection occur
immediately (in the OR)
what is the mechanism by which hyperacute rejection occurs
activation of complement through antibody-mediated interactions– results in inflammation & microvascular thrombosis
how to prevent hyperacute rejection
screening: ABO, HLA
treatment of hyperacute rejection
retransplant
when does acute cellular rejection occur
can occur at any time after transplant but most common in the first 12 months
what is the mechanism by which acute cellular rejection occurs
recognition of foreign antigen leads to T- cell activation
cytokines are produced: CD8, NK and B cells are recruited and causes damage
what is the clinical presentation of acute cellular rejection
depends on organ transplanted:
kidney- abrupt change in SCr
liver- abrupt change in LFTs
heart- decrease in cardiac output or ejection fraction
lung- change in pulmonary function/breathing
management of acute cellular rejection
best treatment is prevention
pharmacologic therapy: corticosteroids (prednisone, methylprednisolone), lymphocyte depletion (antithymocyte globulin, alemtuzumab)
what is the general definition of antibody mediated rejection
a form of allograft rejection triggered by the production of antibodies directed toward donor HLA molecules
in antibody mediated rejection, what do the donor specific antibodies activate
the complement: attaches to and pokes holes into the allograft (membrane attack complex)
what are some therapy targets for antibody mediated rejection
proteasome inhibition (stop plasma cells from making antibodies)
cellular depletion (get effector cells out of circulation)
inhibit signals (IL-6)
inhibit complement cascade
what are the calcineurin inhibitors
cyclosporine and tacrolimus
what is the ultimate action of calcineurin inhibitors
block cytokine transcription by inhibiting calcineurin
true or false: you can substitute sandimmune for neoral or gengraf
FALSE: the bioavailability of cyclosporine depends on the formulation and you CANNOT substitute
different brand names of cyclosporine and their absorption
sandimmune: high variability. <10% up to 89% bioavailable. depends on enterohepatic recirculation
neoral, gengraf: 30% bioavailable, more consistent absorption, less dependent on bile
effects of food on the calcineurin inhibitors
high fat meal decreases absorption
cyclosporine causes drug interactions because of being _____ substrate
CYP3A4
cyclosporine elimination is extensive in the ____
bile
cyclosporine adverse effects
think c for cyclosporine and c for cosmetic: acne, hirsutism, gingival hyperplasia
as well as:
nephrotoxicity and neurotoxicity- dose limiting
hypertension
hyperlipidemia
hyperglycemia
CYP3A4 inhibitors will ______ cyclosporine levels. is this reaction immediate or delayed?
increase
interaction happens quick because the protein is already there and you just inhibit it
CYP3A4 inducers will ____ cyclosporine levels. is this reaction immediate or delayed?
decrease
interaction is delayed because you have to make new proteins, takes a couple weeks
cyclosporine interaction with OATP substrates
cyclosporine inhibits OATP and increases the OATP substrate concentration
IV doses of cyclosporine are ____ PO doses
1/3
counseling point for cyclosporine
maintain consistent administration with meals
don’t care if you eat or not just do it consistently
TDM for cyclosporine
narrow therapeutic window: methods are trough, AUC, peak monitoring
with extensive and intermediate CYP3A5 metabolizers, _____ doses of tacrolimus are needed
increased
with poor CYP3A5 metabolizers, _____ doses of tacrolimus are needed
standard
tacrolimus absorption
bioavailability 30%, erratic and variable absorption– decreased with high fat meal
tacrolimus side effects
nephrotoxicity (dose limiting): hyperkalemia, hypomagnesemia
neurotoxicity (dose limiting): headache, tremor, insomnia, seizure
hypertension
hyperlipidemia
hyperglycemia
pruritis
cosmetic- alopecia
tacrolimus drug interactions
decreased GI absorption with cationic compounds
CYP3A4 inducers decrease TAC levels, inhibitors increase TAC levels
IV doses of tacrolimus are ___ of PO doses
1/3
TDM for tacrolimus
narrow therapeutic window: trough concentrations are easiest to measure but may not correlate with efficacy/toxicity
which transplant drugs are purine analogs
mycophenolic acid, azathioprine, mercaptopurine
what is the basic mechanism of mycophenolic acid
to block T and B cell proliferation and activity. does so by inhibiting IMPDH to block purine nucleotide synthesis
adverse effects of mycophenolic acid
bone marrow suppression and GI toxicity are dose limiting
progressive multifocal leukoencephalopathy (PML)
drug interactions with mycophenolic acid
decreased GI absorption with cationic compounds, decreased elimination with acyclovir and ganciclovir
mycophenolate mofetil dose of 500 mg correlates to mycophenolic acid dose of ____
360 mg
TDM for mycophenolic acid
controversial; AUC monitoring most widely used
bioavailability of mycophenolate
depends on formulation:
MMF 94%; rapidly converted to MPA
MPA 72%: protected by enteric coating
mechanism of azathioprine and mercaptopurine
block de novo and salvage pathways for purine synthesis; suppresses T>B cells, anti-inflammatory
2 enzymes responsible for azathioprine/mercaptopurine metabolism
TPMT to inactive & active metabolites
xanthine oxidase to inactive metabolites
azathioprine/mercaptopurine side effects
bone marrow suppression (dose limiting)
GI toxicity (less than MPA)
alopecia, hepatitis, pancreatitis
azathioprine/mercaptopurine drug interactions
xanthine oxidase inhibitors (allopurinol, febuxostat) decrease metabolism and increase toxicity of azathioprine. requires decrease azathioprine doses to 1/3 or 1/4 of the usual dose but generally avoid the combination
TDM for azathioprine/mercaptopurine
TPMT activity
monitor CBC
name the pyrimidine synthesis inhibitor
leflunomide
leflunomide mechanism
ultimately blocks T and B cell proliferation and activity by blocking pyrimidine nucleotide synthesis
leflunomide side effects
bone marrow suppression, GI side effects, alopecia
leflunomide drug interaction
bile acid sequestrants
leflunomide PO dosing requires ____
loading dose
what drugs are the mTOR inhibitors
sirolimus and everolimus
mechanism of the mTOR inhibitors
inhibiting mTOR which is what moves the cell from G1 to S phase. ultimate activity is blocking response to IL-2 and effects on cell cycle
mTOR inhibitors: how does their absorption compare
sirolimus is erratic and variable (14% solution to 27% tablets)
everolimus is more consistent (30%)
both are impacted by high fat meals, highly distributed in WBCs
mTOR inhibitor side effects
bone marrow suppression
GI toxicity
wound dehiscence
hyperlipidemia
proteinuria
pulmonary infiltrates
mTOR inhibitors drug interactions
CYP3A4 inducers decrease
CYP3A4 inhibitors increase
with cyclosporine, you have to take sirolimus 4 hours after it
mTOR inhibitors: which one requires a loading dose
sirolimus