Transpiration and Translocation Flashcards

1
Q

What is the benefit of xylem tissue being dead?

A

It means there is little resistance to water movement.

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2
Q

Describe the structure of a xylem.

A

Made of dead cells joined end to end, forming a hollow tube. Strengthened with lignin.

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3
Q

Explain cohesion - tension theory.

A

The theory that states movement of water in plants caused by a continuous pull of water up from the roots and out to the leaves.

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4
Q

Define cohesion.

A

Water molecules stick to each other due to attraction formed by hydrogen bonds.

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5
Q

Define adhesion.

A

Water molecules stick to other things (such as the xylem vessel).

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6
Q

Describe how cohesion/tension work in a plant.
Explain in terms of pressure.

A

Water molecules stick to the xylem vessel resisting the force of gravity. As water is constantly being lost in evaporation from leaves, this draws more water up from the roots, through the xylem. This forms a continuous unbroken column called the transpiration pull.
Transpiration puts the xylem under a negative pressure.

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7
Q

Describe how water moves through leaves.

A

Water evaporates from mesophyll cells and lands in air spaces between leaves. This lowers the water potential in cells and raises it in the air spaces. In turn, cells take water from other cells by osmosis to raise their water potential.

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8
Q

How is water lost from the plant through stomata?

A

Humidity of atmosphere is usually less than in the plant so water potential lower in atmosphere. This draws water out of the plant by diffusion through the stomata in evaporation.

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9
Q

How does increased wind speed affect transpiration?

A

Causes air molecules to be blown away from leaves, resulting in a steeper concentration gradient and a faster diffusion rate.

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10
Q

How does increased temperature affect transpiration?

A

Increase in temperature raises the kinetic energy of particles, so they move faster.

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11
Q

How does increased humidity affect transpiration?

A

Flattens the concentration gradient, so diffusion is slower.

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12
Q

How can sunlight lead to water loss?

A

Sunlight means more photosynthesis. This means there are more waste gases that need to be lost from the plant, so stomata open. But if more stomata are open then more water can be lost.

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13
Q

Give 3 reasons why plants need water.

A

As a metabolite
To keep leaves cool
For use in hydrolysis

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14
Q

How can changes in tree trunk diameter support cohesion-tension theory?
Explain what would happen if transpiration is high and low.

A

When transpiration is at its highest, there is more tension in the xylem. This pulls the walls of the xylem inwards and causing the tree trunk to shrink.
When transpiration is low, there is less tension in the xylem. Therefore, the walls push outwards, causing the diameter to increase in length.

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15
Q

What happens if the xylem column breaks?

A

Air enters, so water molecules can no longer form a tight stick to the walls.

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16
Q

As water does not leak out when they xylem column breaks, how does this support cohesion tension theory?
Compare this to if pressure were to maintain the transpiration pull.

A

Instead air is drawn in.
If the system was under pressure instead, then water would leak.

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17
Q

Describe phloem structure.

A

Live cells form columns that are separated by sieve plates.

18
Q

What is translocation?

A

Movement of nutrients around a plant.

19
Q

What is a source?

A

Where organic molecules are produced.

20
Q

What is a sink?

A

Where organic molecules are used.

21
Q

Why is translocation bidirectional?

A

Because sources and sinks can be found anywhere in the plant so nutrients must be able to travel in both directions.

22
Q

Name the 1st stage in translocation.
Explain what this is.

A

Phloem loading.
Transfer of sucrose into sieve plates from photosynthesising tissue.

23
Q

Where are companion cells found?

A

Cells found directly next to the phloem.

24
Q

How are companion cells and phloem separated?

A

By plasmodesmata, which links the cytoplasms of the 2 together.

25
Q

Describe the mechanism of how sucrose is transferred into companion cells from phloem.

A

Sucrose enters companion cells by facilitated diffusion through a co transporter alongside H+ ions.

26
Q

Describe how sucrose is moved from photosynthesising tissue towards sieve tubes.

A

Sucrose diffuses from a high concentration in photosynthesising cells towards a low concentration around sieve tubes.

27
Q

Describe how ATP is used during translocation.

A

ATP needed to pump H+ ions from companion cells to the space between the cell wall.

28
Q

Name the 2nd stage of translocation.

A

Mass flow

29
Q
  1. In mass flow, by what transport process is sucrose loaded into sieve tubes?
    How does this change the water potential of sieve tubes?
A

Sucrose enters sieve tubes by active transport.
Water potential lowers in sieve tube elements.

30
Q
  1. In translocation, how does hydrostatic pressure in the sieve tube change after water potential has dropped?
    Where does water enter sieve tubes from and by what transport process, to try and raise water potential?
A

Drop in water potential leads to water entering sieve tubes by osmosis from the xylem. Therefore, hydrostatic pressure increases.

31
Q
  1. In translocation, where does water move from/to in terms of hydrostatic pressure?
A

Water moves from a region of high hydrostatic pressure at the source to a low hydrostatic pressure at the sink.

32
Q
  1. At respiring cells in the sink, how does sucrose concentration in these cells change and by what transport process will cause more to enter? How does this affect their water potential?
A

Sucrose used in respiration or to make starch. Decreased sucrose concentration causes more to be actively transported in, decreasing water potential.

33
Q
  1. As water potential in respiring cells is now lower, where does water move from/to?
    How does this change hydrostatic pressure in sieve tubes?
A

Lower water potential causes water to move into these respiring cells from sieve tubes, by osmosis. This reduces hydrostatic pressure in sieve tubes.

34
Q
  1. Why is hydrostatic pressure high at the source and low at the sink?
A

Water enters sieve tubes at sources and leaves at sinksw.

35
Q

Describe the overall flow of sucrose solution through a plant.

A

Sucrose solution moves from region of high hydrostatic pressure at the source to a low region of hydrostatic pressure at the sink.

36
Q

By what transport process is sucrose removed from sieve tube elements to sinks or other sources?

A

Active transport

37
Q

How are tree ringing experiments set up when investigating translocation?

A

Bark and phloem removed, leaving the xylem stem.

38
Q

How do ringing experiments for translocation show evidence for it?

A

Sugars accumulate in the region above where the bark/phloem are removed and it swells.

39
Q

What is a tracer experiment?

A

Uses radioactive 14CO2 isotopes to see how sugars are moved through a plant.

40
Q

How do we set up a tracer experiment?

A

We grow a plant in an atmosphere rich with 14CO2.

41
Q

Describe what we would do to see sugars move through a plant in a tracer experiment and how we would interpret results.
1. What imaging technique do we use?
2. What do the blackened regions on the image produced show?
3. Which part of the plant do the blackened regions represent?
4. What do the non blackened regions show?

A
  1. Place a cross section of a plant in some x ray film.
  2. Film blackens where it has been exposed to radiation by sugars containing the radioactive 14C isotope.
  3. Blackened regions correspond with phloem tissues.
  4. Non blackened regions do not carry sugars.