translation stoppers Flashcards
Streptomycin
binds to 30S subunit to disrupt initiation of translation
Binds where fmet-tRNA is
Clindamycin & Erythromycin
-bind to 50S subunit to disrupt translocation of ribosome
what does clindamycin used to treat?
used to treat pertussis
Shiga Toxin & Ricin
-binds to 60S subunit to disrupt elongation
Diphtheria toxin
-inactivates EF2-GTP and inhibits elongation
Tetracyclines
bind to the 30S subunit to disrupt elongation
Blocks aminoacyl-tRNA to ribosomal complex
Puromycin
causes premature chain termination in both prok/euks
Forms a puromyclated chain at 3’ end of aminoacylated-tRNAs which leads to premature chain release
Stops ribosome from functioning
Peptidyl transferase activity
-housed in large subunits
Chloramphenicol
inhibits peptidyl transferase
Prokaryotes & mitochondria
Cycloheximide
inhibits peptidyl transferase
Eukaryotes