Translation

Question 1

Stages of translation

Answer 1

-tRNA charging
-Initiation
-Elongation
-Termination

Question 2

Features of genetic code

Answer 2

-Degenerate
-almost universal
-3 bases to one a
-nonoverlapping
-initiation codons AUG
-Stop codons

Question 3

Formation of peptide bond

Answer 3

N terminus to c terminus

Question 4

TRNA structure

Answer 4

Question 5

Which enzymes charges tRNA molecule

Answer 5

aminoacyl tRNA synthetase (uses ATP)

Question 6

Translation initiation in bacteria

Answer 6

Question 7

Shine Dalgarno sequence

Answer 7

AGGAGG

Question 8

Translation initiation in Eu

Answer 8

Question 9

Nonsense mediated decay

Answer 9

Question 10

Elongation in bacteria (translation)

Answer 10

-fMet carrying tRNA moves into P site of ribisome
-another tRNA enters A site
-GTP is cleaved into GDP and a peptide bond forms between the amino acids in A and P site
-Ribisome moves down to next codon on mRNA
-tRNA in P site releases aa and translocates into E site where it moves into cytoplasm
-tRNA in A site moves into P site and A site is now open for a new tRNA

Question 11

Ribisome sites

Answer 11

E-exit site
P-peptidyl site
A-aminoacyl site

Question 12

Mechanism of termination of translation

Answer 12

Question 13

Differences between Pro and Eu translation

Answer 13