Transgenic animals

Question 1

Nuclear transfer

Answer 1

replaced nucleus of egg cell witj edited somatic cell, develop to blastocyst in vitro and then put into mother

Question 2

DNA microinjection

Answer 2

inject DNA into male pronucleus, egg added back into the mother

Question 3

Embryonic stem cell

Answer 3

take embryonic cells from blastocyst, introduce vector via electroporation
screen and insert into blastocyst

Question 4

retrovirus mediated gene transfer

Answer 4

virus that carries RNA
8-cell stage embryos are infected with retrovirus carrying transgene

Question 5

Cattle offspring

Answer 5

SRY gene into chromosome 17, male traits expressed

Question 6

fat fish

Answer 6

tiger puffer = no appetite, disrupt leptin receptor
red snapper = myostatin, protein that suppresses muscle growth

Question 7

galsafe pig

Answer 7

hyper acute rejection: preformed antibodies to galactose alpha 1, 3 galactose sugar -> remove GGTA1
doesn’t address porcine endogenous retrovirus

Question 8

wool production

Answer 8

CysE and CysK genes isolated from E.coli inserted into sheep
Encodes for enzymes which synthesise cysteine from sodium sulphite
more wool

Question 9

dragline silk

Answer 9

express dragline silk gene in mammalian cells (goat), in milk

Question 10

transgenic pigs to eat

Answer 10

Desaturase: makes more omega 3 fatty acid from omega 6 fatty acids