Transfusion Products

Question 1

Total volume in packed red cell concentrate

Answer 1

220-340ml

contains HB > 40g and 55-30ml plasma

Question 2

Shelf live of packed red cells

Answer 2

35 days

Question 3

Storage temp of pack red cells

Answer 3

4 degrees

Question 4

why give pack red cells

Answer 4

to increase oxygen carrying capacity of blood

Question 5

platelets transfusions are given

Answer 5

primarily to prevent rather than treat Haemorrhage

Question 6

platelet storage

Answer 6

Shelf life up to 5 days (7 if bacteriologically screened) at 22 degrees C on an agitator. NEVER store in fridge as this causes irreversible aggregation. If unused must be returned to HTL as unit can be reissued

• much higher risk of infection for platelets as stored at higher temp

Question 7

Platelets are indicated

Answer 7

Bone marrow failure eg bleeding or to improve platelet count
Disseminated Intravascular Coagulation
Massive transfusion leading to dilutional thrombocytopaenia

Question 8

Fresh frozen plasma is indicated in

Answer 8

Coagulation factor deficiency where no factor concentrate available
Acute DIC with evidence of bleeding
Thrombotic thrombocytopenic purpura (bleeding into tissues)

• Should not be used for management of hypovoaemia*
• *FFP not a way of giving plasma.
• *FFP used to give clotting factors

Question 9

How is FFP made

Answer 9

Remove from whole blood donation and frozen within 8 hours

Question 10

How is FFP stored

Answer 10

-25 degrees C for 2 years

Question 11

Dose of FFP

Answer 11

12-15ml/kg

Question 12

FFP conditions for use

Answer 12

thaw prior to use
best used immediately
can be stored at 4 degrees for 24 hour

Question 13

How is cryoprecipitate prepared

Answer 13

from FFP by slow thawing at 4-6 degrees

pooled from4-5 donors

Question 14

Cryoprecipitate contains

Answer 14

Fibrinogen
Factor 8
vonWillibrand Factor

Question 15

which transfusion product has greatest risk of acute transfusion reactions

Answer 15

FFP and Cryprecipitate

Question 16

Cryoprecipitate is indicated

Answer 16

to increase fibrinogen levels eg in massive transfusion and disseminated intravascular coagulation

Question 17

Blood conservation strategies

Answer 17

**Restrictive transfusion protocol (HB

Im sure I did this

Question 18

Types of transfusion

Answer 18

Allogeneic - derived from another human

Autologous - self derived either by preoperative donation or intra operative cell slavage

Question 19

How should cryoprecipitate be handled

Answer 19

Once thawed cannot be refrozen as coagulation factors start to deteriorate. If unused return to HTL for destruction

Question 20

Cryoprecipitate storage

Answer 20

stored frozen at -30 degrees for up to 2 years. Once request made thawed rapidly at 37 degrees

Question 21

why should unused red cells out of refrigeration and not transfused within 4 hours be returned to HTL

Answer 21

because of increased risk of bacterial proliferation and red cell metabolism as pack warms to room temp