Transcriptional regulation Flashcards
role of sigma unit of RNA pol
promoter recognition
role of alpha subunits of RNA pol
assembly, activation
role of beta subunits of RNA pol
catalysis, termination
role of omega subunit of RNA pol
assembly, folding, required for some genes
RNA pol I transcribes…
ribosomal RNA genes
RNA pol II transcribes…
protein coding genes (mRNA)
small nuclear RNA (snRNA)
RNA pol III transcribes…
transfer RNA (tRNA), rRNA and snRNA
TFIID is composed of…
TATA binding protein (TBP) + TBP-Associated Factors (TAFs)
Role of TFIIE, F, H, J, K
Interaction with D-A-B complex and recruitment of polymerase II
Blocks non-specific binding of polymerase II to DNA
Promoter clearance
Helicase
Processivity, elongation
Transcription-coupled DNA repair
What is the operon?
cluster of genes transcribed by the same
promoter that gives rise to a polycistronic mRNA -
genes usually related
Lactose is an inducer- true or false?
true
What is the role of CRP/CAP?
catabolite repression- cAMP binds to CRP permitting DNA binding
Define diauxie
The phenomenon whereby a bacterium, when
provided with a mixture of sugars, uses up one sugar
before beginning to metabolize the second sugar.
- intro to genetics, brown
Role of histone acetylase (HAT) in transcription initiation
ATP-dependent chromatin modification complexes
that transfer an acetyl group to histones to expose DNA for transcription to start.
Give as many examples of steroids as possible.
Estrogen androgen corticosteroids retinoids vitamin D ecdysone Fatty acids thyroid hormone
