Transcription/Translation Flashcards
DNA pol alpha
contains primase
initiates dna repair
no proof reading
eu
DNA pol beta
repair
no proofreading
eu
DNA pol gamma
mitochondrial
proof reads
eu
DNA pol delta
elongate Okazaki fragments of lagging strand
proof reads
eu
DNA pol epsilon
elongate leading strand
proof reads
eu
RNA pol 1
nucleus
rRNA
RNA pol 2
nucleoplasm
mRNA & snRNA
RNA pol 3
nucleoplasm
tRNA & 5S rRNA
protein to be secreted
signal seq on its amino-terminal end recognized by the SRP (signal recog particle) in the cytoplasm
lysosomal degradation of proteins
non-selective
mediated by proteases = cathepsins
mostly degradation of proteins ingested from extracellular environment
cytosolic degradation
selective, ATP-dependent, mor sig
degradation of short-lived, misfolded and truncated proteins
Ubiquitin (at least 4 req for degradation)
ATP-dep proteolysis
N-end rule & PEST internal seq = more rapidly degraded
methionine at N-end = protection
Proteasome - degrades ubiquitylated proteins, ATP dep
initiating amino acid is formylmethionine
prokaryyotic translation
rifampin
interacts with bacterial RNA polymerase, inhibits initiation of transcription
tuberculosis and meningococcal infection
alpha-amanitin
inhibits eukaryotic RNA polymerase II and III at high concentractions
toxic, from mushrooms
transcription
dolichol phosphate used to create glycoproteins here
endoplasmic reticulum
