Transcription & Translation Flashcards
what do you call the making of proteins?
protein synthesis
list the two steps that make up protein synthesis
transcription and translation
My part of the process involves copying a gene’s DNA sequence. what am I?
transcription
what is in RNA that cannot be found in DNA? What does it replace?
Uracil, which replaces thymine
what is a triplet code/codon?
a DNA or RNA molecule that codes for a particular amino acid
my part of the process involves decoding the information for making proteins. What am i?
translation
I can leave the nucleus. what am I?
mRNA
Other than RNA, what other components help in the decoding process?
tRNA (transfer RNA) and the ribosome
where are proteins built?
the ribosome
what are amino acids?
molecules that combine to form proteins
List and explain the steps of translation
Initiation
Ribosome assembles around mRNA to be read and first tRNA (carrying methionine) matches the start codon.
Elongation
This is where the amino acid stage gets longer. The mRNA is read one codon at a time, and the amino acid (aka anticodon) that matches each codon is added. It goes like this:
1. A matching tRNA binds to the codon.
2. the existing amino acid chain is linked onto the amino acid of the tRNA via a chemical reaction
3. the mRNA is shifted one codon over in the ribosome, exposing a new codon for reading.
Termination
this is the stage in which the finished chain is released. It begins when a stop codon enters the ribosome.
List and explain the steps of transcription
Initiation
1. RNA polymerase binds to a sequence of DNA called the promoter (found near the beginning of each gene).
2. Once it’s bound, the RNA polymerase seperares the DNA strand.
Elongation
The RNA polymerase reads the DNA template strand one base at a time. It is through this process that it builds the mRNA, replacing thymine with uracil.
Termination
Sequences called terminators signal that the RNA transcript is complete. Once they are transcribed, the mRNA leaves.
what does the protein do when it is released from the ribosome?
it folds into its functional shape
List the three stop codons
UAA,
UGA
UAG
name the start codon
AUG