Transcription Flashcards

1
Q

Transforming DNA into RNA

A

Transcription

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2
Q

Transcribing DNA to RNA is catalyzed by ______ and occurs in what stage of the cell cycle?

A

RNA polymerase and interphase

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3
Q

What RNA Polymerase makes non coding RNA

A

RNA polymerase 1 and 2

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4
Q

What RNA polymerase makes mRNA

A

RNA Polymerase 3

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5
Q

Where is the location of ribosomal RNA synthesis and transcription on the nucleus

A

Nucleolus

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6
Q

What are the three stages of transcription

A

Initiation
Elongation
Termination

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7
Q

What stage of transcription do proteins assemble at the start of DNA (promoter region)

A

Initiation

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8
Q

Is there more DNA pol or RNA pol in the average cell?

A

RNA pol

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9
Q

What is used as the base sequence for RNA synthesis

A

Antisense strand

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10
Q

What strand of the DNA is identical to the RNA that was synthesized in transcription

A

Sense strand (except for U for T substitution in RNA)

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11
Q

How many bases per second are synthesized for RNA synthesis

A

50-100 bases per second

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12
Q

How many bases per second are synthesized for DNA synthesis

A

1000 bases per second

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13
Q

RNA synthesis is different from DNA synthesis how?

A

Less fidelity
Slower
Forms transcription bubble during synthesis
Does not need priming

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14
Q

The closest phosphate group to the ribose sugar of a ribonucleoside triphosphate

A

Alpha phosphate

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15
Q

Termination of transcription by a transcription complex factor, Helicase enzyme stopping polymerase at cytosine rich sites in DNA

A

Rho

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16
Q

What is Rho independent termination?

A

GC rich bases are transcribed into RNA creating a hairpin double stranded structure,slowing elongation and reaching the AT rich region where the elongation complex dissociates

17
Q

What does a polyA site/signal cause

A

When pol 2 encounters a poly A tail termination of transcription is activated