Transcription Flashcards
What happens during transcription?
An mRNA copy of a gene is made from DNA
Where does transcription happen in eukaryotic cells?
Nucleus
Where does transcription happen in prokaryotic cells?
Cytoplasm
When does transcription start?
When RNA polymerase attaches to DNA double-helix at beginning of a gene
What is the 1st stage of transcription?
Enzyme help case breaks bonds between bases in a specific region of double-stranded nucleus
This causes the 2 strands to separate
What is the 2nd stage of transcription?
RNA polymerase binds to a base sequence called promoter region
This determines which way the RNA polymerase faces and hence which region is used as the template
What is the 5th stage of transcription?
As RNA polymerase passes over the DNA bases it forms a complementary mRNA strand from free RNA nucleotides in the nucleus
What happens during stage 4 of transcription?
As the mRNA strand is produced, the 2 strands of DNA start to recoil behind it
What happens during stage 5 of transcription?
When a terminator region is reached, the DNA is no longer copied
The mRNA is now ready for translation
What is the role of the helical enzyme?
To break the hydrogen bonds between bases in DNA to form 2 separate strands
What is the role of RNA polymerase?
Joins RNA nucleotides together via a condensation reaction to form a strand of mRNA