Transcription

Question 1

Describe initiation

Answer 1

TIC;

Binding of RNA polymerase;

DNA double helix unwind;

2 DNA strands unzip at transcription start site

Question 2

Describe elongation

Answer 2

RNA poly moves along the DNA template strand;

Unwind and unzip double helix;

10-20 DNA bases for complementary base-pairing with free ribonucleoside triphosphates

RNA poly. catalyses the formation of phosphodiester bond

Question 3

Describe termination

Answer 3

RNA poly transcribes a terminator seq, AAUAAA

RNA poly dissociate from DNA;

DNA fully rewind while RNA dissociate from the template strand;

pre-mRNA