TRANSCRIPTION Flashcards
What is a gene
a lenth of DNA that codes for one or more polypeptide chain
define the term genome
is the entire DNA sequence of that organism.
Consist of about 3 billion nucleotide base pairs
genes code for polypeptides such as what?
- haemoglobin
- collagen
- antibodies
- actin and myosin in muscel cells
- channel proteins
- electron carriers
- enzymes
what is the genetic code
sequence of nucleotide bases that code for the construction of proteins e..g.haemoglobin
name 4 characteristics of the genetic code
- Its a triplet codes,20 amino acids code for proteins sythesis,43 =64 codes this is more than enough,a double or single code wont be enough
- Its a degenerate code,all amino acids have more than one code expect for methionine
- Some codes indicate a stop e.g. methionine,which means the end of a polypeptide chain
- Genetic code is widespread but not universal
why is it useful that the genetic code is widespread
Can be used in genetic engeenering,as we can tranfare a gene from one organism into another and it will produce the same protein e.g. insulin can be produced im large quantities by bacteria
outline the mechanism of transciption
- Hydrogen bonds between base pairs are broken by DNA helicase,this caused the section to be trasciped to unwind
- Free activated nucleotides bind with hydrogen bonds to their complementary bases e.g. U-A, G-C, T-A,this is catalysed by RNA polymerase using energy from extra phosphates on nucleotides
- mRNA is released from the DNA and passes out of the nucleus through nuclear pores in the nuclear envelop and it then attaches to a ribosome in the cytoplasm
what is the role of RNA Polymerase
- Catalyses the condensation reaction that occur between adjacent RNA nucleotides
- Checks that the bases have paired up correctly