Transcription

Question 1

What is the promoter site?

Answer 1

Sequence of nucleotides (40 BP) that occurs upstream of each gene

The promoter site is essential for the initiation of transcription.

Question 2

What is the TATA site?

Answer 2

Component of the promoter site

The TATA site is crucial for the binding of transcription factors.

Question 3

What is the terminator site?

Answer 3

Region of nucleotides that signals the end of the gene (not part of gene)

The terminator site ensures transcription stops at the correct location.

Question 4

What is the role of transcription factors?

Answer 4

Binds to chromosome just upstream of the promoter

Transcription factors are proteins that facilitate the binding of RNA polymerase.

Question 5

What is the function of RNA polymerase?

Answer 5

Enzyme that makes RNA polymer and transcribes the gene

RNA polymerase is essential for the synthesis of RNA from the DNA template.

Question 6

Describe the process RNA polymerase follows during transcription.

Answer 6

Moves down gene, separates strands, adds free RNA nucleotides to one of the exposed strands (3’-5’)

This process involves the elongation phase of transcription.

Question 7

Fill in the blank: The promoter site is located _______ of each gene.

Answer 7

upstream

The upstream location is critical for the regulation of gene expression.