Transcription Flashcards
Is RNA double stranded or single stranded .
Single stranded
How many ways can RNA fold into stable DNA structure
Three
What forms double stranded stem & hairpin loops
Conventional b.p interactions
What establishes contacts that determine the tertiary structure of RNA molecules
Non-conventional interactions
Which direction does transcription occur
From 5’ - 3’
Does transcription have to have a primer to initiate
No
How does the cell know which part of the genome to transcribe?
Gene encodes signals for transcription
What does the promoter sequence control
Transcription initiation and the transcription start site (TSS)
What does the terminator sequence lead to?
Dissociation of polymerase
What enzyme catalyses transcription
RNA Polymerase
Which enzyme recognises the promoter, starts transcription, open the DNA duplex, form a transcription bubble & elongates RNA strand along gene until the termination sequence
RNA polymerase
What 4 subunits make up E.coli RNA
Alpha, beta, beta’ & sigma factor
Which core E.coli RNA subunit binds regulatory sequences
Alpha
Which core E.coli RNA subunit forms phosphodiester bonds
Beta
Which core E.coli RNA subunit binds the DNA template
Beta’
