Transciption Flashcards

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1
Q

what does transcription do?

A

create a mRNA copy of a gene from a DNA template.

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2
Q

where does transription take place inn eukaryotic cells?

A

in the nucleus

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3
Q

what enzyme is involved in the breaking of the hydrogen bonds?

A

RNA polymerase

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4
Q

what is the first step of transcription?

A

RNA polymerase binds to DNA

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5
Q

what is the seconds step of transcription?

A

The hydrogen bonds between the DNA bases break, and the two strands of the double helix separate.

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6
Q

what is the third step of transcription?

A

The antisense strand acts as the template for mRNA synthesis.

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7
Q

what is the fourth step of transcription?

A

Free RNA nucleotides align with the DNA template through complementary base pairing.

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8
Q

what are the bonding pairs?

A

In the RNA molecule, uracil pairs with adenine, while adenine pairs with thymine, and cytosine pairs with guanine

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9
Q

what is the fifth step in the transcription?

A

RNA polymerase catalyses the formation of phosphodiester bonds between adjacent RNA nucleotides.

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10
Q

what is the sixth step in transcription?

A

A complementary mRNA strand is formed, carrying the same base sequence as the DNA sense strand.

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11
Q

what is the seventh step in transcription?

A

The process ends when RNA polymerase reaches a stop codon, detaches from DNA and terminates transcription.

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12
Q

what is the eighth step in transcription?

A

mRNA is released, detaches from DNA, and DNA rewinds into its double helix structure.

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13
Q

doe transcription differ in eukaryotic and prokaryotic ?

A

yes

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14
Q

how does transcription work in eukaryotic ?

A

pre-mRNA is spliced after transcription to remove introns before it is transported to the cytoplasm.
both introns (non-coding regions) and exons (coding regions) of DNA are transcribed into the initial mRNA transcript (pre-mRNA).
This pre-mRNA undergoes RNA splicing, a process where introns are removed, and exons are joined together.
This produces mature mRNA consisting of only exon sequences.
This mature mRNA then travels out of the nucleus for translation.

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15
Q

how does transcription work in prokaryotic?

A

mRNA is directly produced from transcription without any splicing.

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