Transciption Flashcards
what does transcription do?
create a mRNA copy of a gene from a DNA template.
where does transription take place inn eukaryotic cells?
in the nucleus
what enzyme is involved in the breaking of the hydrogen bonds?
RNA polymerase
what is the first step of transcription?
RNA polymerase binds to DNA
what is the seconds step of transcription?
The hydrogen bonds between the DNA bases break, and the two strands of the double helix separate.
what is the third step of transcription?
The antisense strand acts as the template for mRNA synthesis.
what is the fourth step of transcription?
Free RNA nucleotides align with the DNA template through complementary base pairing.
what are the bonding pairs?
In the RNA molecule, uracil pairs with adenine, while adenine pairs with thymine, and cytosine pairs with guanine
what is the fifth step in the transcription?
RNA polymerase catalyses the formation of phosphodiester bonds between adjacent RNA nucleotides.
what is the sixth step in transcription?
A complementary mRNA strand is formed, carrying the same base sequence as the DNA sense strand.
what is the seventh step in transcription?
The process ends when RNA polymerase reaches a stop codon, detaches from DNA and terminates transcription.
what is the eighth step in transcription?
mRNA is released, detaches from DNA, and DNA rewinds into its double helix structure.
doe transcription differ in eukaryotic and prokaryotic ?
yes
how does transcription work in eukaryotic ?
pre-mRNA is spliced after transcription to remove introns before it is transported to the cytoplasm.
both introns (non-coding regions) and exons (coding regions) of DNA are transcribed into the initial mRNA transcript (pre-mRNA).
This pre-mRNA undergoes RNA splicing, a process where introns are removed, and exons are joined together.
This produces mature mRNA consisting of only exon sequences.
This mature mRNA then travels out of the nucleus for translation.
how does transcription work in prokaryotic?
mRNA is directly produced from transcription without any splicing.