Topic 9 Flashcards
What is thymine replaced with in rna
Uracil.
Instead of a to t
It’s a to u
What direction is the dna template read by tRNA
3 to 5
Rna made 5-3
Lecture I didn’t watch
Ok
What are exons in dna
The protein coding region
The region that code for the amino acids
Are introns in premrna
Mature mRNA?
Yes
No
What gets transcribed in transcription
The introns
The exons (protien coding regions)
5-‘ UTR and 3’UTR (untranslated region)
Start and stop codons
Do prokaryotes have intron
No
What do intron begins and end with
GU at 5’ splice site
AG at 3’ splice site
Where does transcription of dna start
At the end of the promoter (right after the promoter) this is the +1 site
Anything before the +1 site is -1 -2 -3
Anything after the +1 site is +2 +3 ….
What is upstream what is meant by downstream
Upstream: toward 5 prime end
Downstream: toward 3 prime end
What is the promoter
Upstream of the sense 5-3 strand
What is the antisense strand
The strand that is the bottom strand (3-5)
What strand does rna polymerase read
The antisense strand
The mRNA sequence is the exact same as the ____ strand other than the fact the t is ____
Sense
U
Wherever 5 end of mRNA is, is where the promoter is
Ok
What is Q PCr
A method of PCr that lets us quantify the amount of dna in the original template dna sample
Also used measure sysnthesis of mRNA (is gene is off or on and at what level)
What is the ct number in Q PCr
The replicationcycle number at which the fluorescence level crosses the thresh holds
What ever has more dna in Q PCr has ____ ct value
Lower
Reached the threshold quicker
Qrt PCr in pics
Ok
What direction is the tRNA molecule
What direction on the mRNA does the ribosome move
3-5
5-3
How do the ribosome know where to start translating on the mRNA
The shine dalgarno (prokaryotes)
The kozak sequence (eukaryotes)
Both sequences are upstream of the start codon
Between the +1 site and the start codon
What is a conservative and non conservative missense mutations
Conservative is when is makes chemically similar amino acid
Non conservation is when it makes chemically diff amino acid
What is a western blot
Used to find a specific protien from a mix of protiens, also determines the level of expression and size of the protien
What happens in western blot analysis
Boil the samples after gel electrophoresis and add detergent to separate the protiens based only on the number of amino acids
Use probes (antibodies) to bind specific proteins. The secondary antibody bind the primary antibody to the specific protien
