Topic 9

Question 1

What is thymine replaced with in rna

Answer 1

Uracil.

Instead of a to t

It’s a to u

Question 2

What direction is the dna template read by tRNA

Answer 2

3 to 5
Rna made 5-3

Question 3

Lecture I didn’t watch

Answer 3

Ok

Question 4

What are exons in dna

Answer 4

The protein coding region

The region that code for the amino acids

Question 5

Are introns in premrna
Mature mRNA?

Answer 5

Yes
No

Question 6

What gets transcribed in transcription

Answer 6

The introns
The exons (protien coding regions)
5-‘ UTR and 3’UTR (untranslated region)
Start and stop codons

Question 7

Do prokaryotes have intron

Answer 7

No

Question 8

What do intron begins and end with

Answer 8

GU at 5’ splice site
AG at 3’ splice site

Question 9

Where does transcription of dna start

Answer 9

At the end of the promoter (right after the promoter) this is the +1 site

Anything before the +1 site is -1 -2 -3
Anything after the +1 site is +2 +3 ….

Question 10

What is upstream what is meant by downstream

Answer 10

Upstream: toward 5 prime end
Downstream: toward 3 prime end

Question 11

What is the promoter

Answer 11

Upstream of the sense 5-3 strand

Question 12

What is the antisense strand

Answer 12

The strand that is the bottom strand (3-5)

Question 13

What strand does rna polymerase read

Answer 13

The antisense strand

Question 14

The mRNA sequence is the exact same as the ____ strand other than the fact the t is ____

Answer 14

Sense
U

Question 15

Wherever 5 end of mRNA is, is where the promoter is

Answer 15

Ok

Question 16

What is Q PCr

Answer 16

A method of PCr that lets us quantify the amount of dna in the original template dna sample

Also used measure sysnthesis of mRNA (is gene is off or on and at what level)

Question 17

What is the ct number in Q PCr

Answer 17

The replicationcycle number at which the fluorescence level crosses the thresh holds

Question 18

What ever has more dna in Q PCr has ____ ct value

Answer 18

Lower
Reached the threshold quicker

Question 19

Qrt PCr in pics

Answer 19

Ok

Question 20

What direction is the tRNA molecule
What direction on the mRNA does the ribosome move

Answer 20

3-5
5-3

Question 21

How do the ribosome know where to start translating on the mRNA

Answer 21

The shine dalgarno (prokaryotes)
The kozak sequence (eukaryotes)

Both sequences are upstream of the start codon

Between the +1 site and the start codon

Question 22

What is a conservative and non conservative missense mutations

Answer 22

Conservative is when is makes chemically similar amino acid

Non conservation is when it makes chemically diff amino acid

Question 23

What is a western blot

Answer 23

Used to find a specific protien from a mix of protiens, also determines the level of expression and size of the protien

Question 24

What happens in western blot analysis

Answer 24

Boil the samples after gel electrophoresis and add detergent to separate the protiens based only on the number of amino acids

Use probes (antibodies) to bind specific proteins. The secondary antibody bind the primary antibody to the specific protien