Topic 9 Flashcards

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1
Q

What is thymine replaced with in rna

A

Uracil.

Instead of a to t

It’s a to u

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2
Q

What direction is the dna template read by tRNA

A

3 to 5
Rna made 5-3

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3
Q

Lecture I didn’t watch

A

Ok

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4
Q

What are exons in dna

A

The protein coding region

The region that code for the amino acids

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5
Q

Are introns in premrna
Mature mRNA?

A

Yes
No

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6
Q

What gets transcribed in transcription

A

The introns
The exons (protien coding regions)
5-‘ UTR and 3’UTR (untranslated region)
Start and stop codons

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7
Q

Do prokaryotes have intron

A

No

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8
Q

What do intron begins and end with

A

GU at 5’ splice site
AG at 3’ splice site

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9
Q

Where does transcription of dna start

A

At the end of the promoter (right after the promoter) this is the +1 site

Anything before the +1 site is -1 -2 -3
Anything after the +1 site is +2 +3 ….

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10
Q

What is upstream what is meant by downstream

A

Upstream: toward 5 prime end
Downstream: toward 3 prime end

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11
Q

What is the promoter

A

Upstream of the sense 5-3 strand

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12
Q

What is the antisense strand

A

The strand that is the bottom strand (3-5)

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13
Q

What strand does rna polymerase read

A

The antisense strand

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14
Q

The mRNA sequence is the exact same as the ____ strand other than the fact the t is ____

A

Sense
U

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15
Q

Wherever 5 end of mRNA is, is where the promoter is

A

Ok

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16
Q

What is Q PCr

A

A method of PCr that lets us quantify the amount of dna in the original template dna sample

Also used measure sysnthesis of mRNA (is gene is off or on and at what level)

17
Q

What is the ct number in Q PCr

A

The replicationcycle number at which the fluorescence level crosses the thresh holds

18
Q

What ever has more dna in Q PCr has ____ ct value

A

Lower
Reached the threshold quicker

19
Q

Qrt PCr in pics

A

Ok

20
Q

What direction is the tRNA molecule
What direction on the mRNA does the ribosome move

A

3-5
5-3

21
Q

How do the ribosome know where to start translating on the mRNA

A

The shine dalgarno (prokaryotes)
The kozak sequence (eukaryotes)

Both sequences are upstream of the start codon

Between the +1 site and the start codon

22
Q

What is a conservative and non conservative missense mutations

A

Conservative is when is makes chemically similar amino acid

Non conservation is when it makes chemically diff amino acid

23
Q

What is a western blot

A

Used to find a specific protien from a mix of protiens, also determines the level of expression and size of the protien

24
Q

What happens in western blot analysis

A

Boil the samples after gel electrophoresis and add detergent to separate the protiens based only on the number of amino acids

Use probes (antibodies) to bind specific proteins. The secondary antibody bind the primary antibody to the specific protien