Topic 7 - Modern Analytical Techniques I Flashcards
What is a mass spectrum?
A bar chart of relative abudnace (y) against m/z (x), used to show the relative abundance of different ions in a sample.
What is used to produce a mass spectrum?
Mass spectrometer.
What is on the y axis of a mass spectrum?
Relative abundance (%)
What is on the x axis of a mass spectrum?
m/z (Mass to charge ratio)
What two things can a mass spectrum be produced for?
- Relative isotopic abundances of one element
* Molecular samples of a compound
What is the molecular ion on a mass spectrum?
- The ion of the entire molecule, with a +1 charge.
* Formed when 1 electron is removed from the molecule.
What is the symbol for the molecular ion on a mass spectrum?
M+ (g)
How do you find the relative molecular mass of a compound from its mass spectrum?
- Find the peak with the highest m/z value (ignoring any small M+1 peaks due to C-13)
- The m/z value is the Mr (assuming the ion has a +1 charge).
What can cause a small peak to the right of the molecular peak on a mass spectrum?
A M+1 peak may be present due to the existence of C-13 isotopes, which increase the m/z by 1.
What is the pattern of fragments on a mass spectrum called?
Fragmentation pattern
What can the fragmentation pattern on a mass spectrum be used for?
Identifying molecules and their structure.
On a mass spectrum, what causes the peaks at smaller m/z values than the molecular peak?
Bombarding electrons make some of the molecular ions to break up into fragments.
On a mass spectrum, when the molecular ion fragments, do both products show up on the mass spectrum?
- No, becauses the ion will break up into an ion and a free radical.
- Only the ion will show up, not the radical.
How might CH3CH2CH3+ fragment?
CH3CH2CH3+ -> CH3CH2• (radical) + CH3+ (ion)
OR
CH3CH2CH3+ -> CH3CH2+ (ion) + •CH3 (radical)
(Only the ions will show up on the mass spectrum)
Explain how you can use a mass spectrum to work out the structure of a molecule.
1) Identify the fragments -> Look at the m/z values and determine what ions could have produced them
2) Piece together the fragments to give a molecule with the correct Mr -> Make sure the m/z values add up to the Mr
Practise using the mass spectrum on pg 100 to work out the structure of the molecule.
Blue box, botom of pg 100 of revision guide.