Topic 5 Photosynthesis Flashcards
Give the structure of a chloroplast
(3)
Thylakoid - stacks of folded membrane
Stroma - fluid centre
Inner & outer membranes - controls what leaves
What stage of photosynthesis are the thylakoid membranes involved in
Light dependant reaction
What part of photosynthesis is the stoma involved in
Light independent reaction
What are the reactants and products used in the LDR
Requires light and water used to create ATP and REDUCED NADP
What are the 4 stages of the LDR
- Photolysis
- Photoionisation of chlorophyll
- Chemiosmosis
- Production of ATP & REDUCED NADP
What is photolysis of water and what are the products used for
When light energy is absorbed by chlorophyll and splits water into oxygen and hydrogen
H+ picked up by NADP forming NADPH used in LIR
e- passed along electron carrier proteins
What is photoionisation of chlorophyll
Light energy absorbed by chlorophyll results in electrons becoming excited and raising energy level so they leave the chlorophyll
Chlorophyll’s then ionised by light
Some energy released electrons used to make ATP
Explain chemiosmosis (5)
- Electrons gained energy left chlorophyll move along series of proteins embedded in thylakoid membrane
- As they move along they release energy which is used to pump protons across the chloroplast membrane
- An electrochemical gradient is created as protons pass through enzyme ATP synthase which results in ATP production
- This electrochemical gradient allows the protons to move via facilitated diffusion back across the membrane
- Protons combine with co-enzyme NADP becoming REDUCED NADP
What is chemiosmosis
Protons move from a high to low concentration gradient
What is the light independent reaction also known as
Calvin cycle
Where does the Calvin cycle occur and what enzymes are present
Occurs in stroma with enzyme rubisco
What reactants does the Calvin cycle need and what are the products
Reactants
Carbon dioxide
Reduced NADP
ATP
Product
Hexose sugar
Explain the Calvin cycle (5)
- Carbon dioxide reacted with ribulose biphosphate (RUBP) 5 carbon molecule to form 2 molecules of 3 Carbon glycerate phosphate (GP)
- Reaction is catalysed by enzyme rubisco
- GP is reduced to triode phosphate (TP) using energy from ATP by accepting a H from reduced NADP
- Some carbon from TP leaves cycle each turn be converted into useful organic substances
- ATP is needed to regenerate RUBP from the 5 carbons left
How many times must the Calvin cycle occur before a hexose sugar is made?
6
What are the 3 limiting factors for photosynthesis
Light intensity
, carbon dioxide concentration and temperature
What are the 4 key stages of aerobic and where do they occur
Glycolysis (cytoplasm)
Link reaction (mitochondrial matrix)
Kerbs cycle (mitochondrial matrix)
Oxidative phosphorylation (mitochondrial inner membrane)
What are the 3 key steps In glycolysis
- Phosphorylation glucose to glucose phosphate using ATP
- Production of triose phosphate
- Oxidation of triose phosphate to produce pyruvate and a net gain of ATP and reduced NAD
Explain the process of glycolysis
- Glucose (6C) is phosphorylated (addition of 2 P) using 2 ATP
- This glucose phosphate then is split into 2 lots of triose phosphate (3C)
- Using ATP and NADH this forms 2 pyruvate (3C)
Give the products of glycolysis
2 x pyruvate
Net gain of 2 ATP (make 4 but use 2)
2 x NADH
Explain the link reaction (2)
- Pyruvate made in glycolysis is oxidised to acetate (2C) with use of NAD which picks up hydrogen becoming Reduced NAD this produces a carbon dioxide
- Acetate then combined with coenzyme A to produce acetyl coenzyme A (2C)
How many times does the link reaction occur for every glucose molecule
2
What are the products of the link reaction
2 x acetyl CoA
2 x CO2 released
2 reduced NAD