Topic 5 - Bacterial cell culture techniques

Question 1

defined media

Answer 1

chemcial composition is known

Question 2

Complex media

Answer 2

composition is unknown

Question 3

enrichment media

Answer 3

favours the growth of select species but not of others, uses blood serum or special growth factors

Question 4

Selective media

Answer 4

enhance the growth of specific species while suppressing the growth of others

Question 5

Differential media

Answer 5

contain dyes chemicals or reagents to help distinguish bacteria, some organisms produces haemolysins to make space around the colony

Question 6

bacteria that can haemolysis

Answer 6

strep. pyogenes and coagulase positive staph. aureus

Question 7

selective and differential media

Answer 7

favour the growth of certain bacteria and inhibit the growth of others

Question 8

Baird parker agar

Answer 8

used for isolation of coagulase positive staph. aureus

Question 9

Eosin methylene blue agar

Answer 9

has high salt which inhibits gram positives, and allows gram negatives to flourish