TOPIC 5 Flashcards
IS A PROCESS WHEREBY ALL FORMS OF MICROBIAL LIFE, INCLUDING BACTERIAL SPORES, ARE KILLED.
STERILIZATION
MAY BE ACCOMPLISHED BY PHYSICAL, OR
CHEMICAL MEANS
STERILIZATION
A PROCESS WHEREBY PATHOGENIC ORGANISMS, BUT NOT
NECESSARILY ALL MICROORGANISMS OR SPORES, ARE DESTROYED.
DISINFECTION
ACCOMPLISHED BY PHYSICAL OR CHEMICAL METHODS.
DISINFECTION
4 METHODS OF STERILIZATION
1.) HEAT
2.) FILTRATION
3.) IONIZATION RADIATION
4.) PARACETIC ACID
Coagulate Proteins
Moist Heat
Most effective method of sterilization
121 C at 15 PSI for
15 minutes - AUTOCLAVE
Infectious medical waste is often sterilized
132 C for 30-
60 minutes (Autoclave)
Quality Control for AUTOCLAVE
Bacillus stearothermophilius
Alternative heating (Kills vegetative cells), incubation
(Spores germinate), heating kills remaining vegetative cells
Fractional
flowing steam 100 C for 30 minutes for
3 successive days.
Tyndallization
75 – 80 C for 2 hours for 3 successive
days.
Inspissation
kills by oxidation
Dry Heat
To sterilizeinoculating loops and needles
Flame
160-180 C for1 1⁄21/2 to 2 hours
Oven
Quality Control of Dry heat
Bacillus subtilis var. niger
- Most common method of treating
infectious waste. - Burned to ashes at 870-980 C.
Incineration
is accomplished using HEPA (High Efficiency
Particulate Air) filters
designed to remove organisms larger than 0-3 um from
isolation rooms,
operating rooms, and biologic safety cabinets (BSCs).
Filtration
*Asbestos Filter
*Membrane Filter
*Method of Choice for antibiotics solutions, toxic chemicals,
radioisotopes,
vaccines and carbs which are all heat sensitive.
FILTRATION
*Used in plastic syringe, catheter or gloves
*Short wavelength, high energy gamma rays,ionizing
gamma radiation
*Used for evacuated tubes
IONIZATION RADIATION
–Most common sterilant
-Quality Control: Bacillus subtilis
Ethylene Oxide (ETO)
-sterilize HEPA filters in BSCs
Formaldehyde vapor and vapor phase hydrogen peroxide
-Sporocidal in 3-10 hours, is used in medical equipment
-Cold sterilization
Glutaraldehyde
-used in surgical equipment
-Cold Sterilization
PARACETIC ACID
100C for 15 minutes, which kills vegetative bacteria
Boiling
Kills milk borne pathogens
Pasteurization
- Long wavelength and low energy
- do not penetrate well,direct surface exposure such as
working surface of BSC (Biological Safety Cabinet)
Non Ionizing Radiation