Topic 4 - Evolution Flashcards
What is symbiosis?
Two organisms helping each other and both benefitting.
What is endosymbiosis?
One organism living inside another and both benefitting.
What is the evidence that prokaryotes existed first?
- prokaryotes are fundamentally simpler in structure than eukaryotes
- mitochondria and chloroplasts display an example of endosymbiosis through their presence within eukaryotic cells (both of these organelles contain DNA that resembles prokaryotes)
What is the evidence that eukaryotes evolved by endosymbiosis?
- chloroplasts and mitochondria have their own DNA that is circular like prokaryotes
- both contain their own ribosomes that are smaller and have similar nucleotide sequences to bacterial ribosomes
- can self-replicate independently to the cell in a process that resembles binary fission
- both have duel membranes, the inner one containing enzymes and transport proteins whilst the outer is like the plasma membrane
What is evolution?
The gradual changes to populations on Earth over long periods of time.
What are the three factors that increase mutation rate?
- ionising radiation
- mutagenic chemicals
- viruses
What is the trend in mutation rate and how does this relate to evolution?
The amount of mutations building up in DNA, RNA or amino acid sequences of proteins is fairly linear with time. This means that if you compare the number of mutations between two species, you can get some idea of when they diverged from a common ancestor.
How does sexual reproduction contribute to genetic variation more than asexual reproduction?
Sexual reproduction provides sources of genetic variation through crossing over, independent assortment, and random fertilisation. Asexual reproduction can only provide genetic variation though mutations.
How do alleles work for genetic diversity?
Alleles are variations of genes, which allows an organism to express one variation whilst still carrying the same genome.
What does comparative genomics describe?
All living organisms have DNA in varying degrees of complexity. Codons (sequence of three nucleotides) code for a specific amino acid, and there are 20 amino acids for all living things. This is compelling evidence for the idea of evolution from common ancestors. Given that mutation is inevitable, as time goes on there is a greater difference in the DNA sequences between organisms. If two organisms have separated relatively recently then there will be less mutations in the DNA, meaning more homology (recent common ancestor). Thus, species that have been separated by a long period of time would have more mutations in DNA, meaning less homology (distant common ancestor).
What are the methods of checking biochemical similarity between species?
DNA-DNA hybridisation, and DNA/rRNA/protein sequencing.
How does DNA-DNA hybridisation work?
DNA from one species is heated, breaking the hydrogen bonds between complementary strands to prompt separation. Single-stranded DNA from the other species is mixed and the mixture is cooled to allow the DNA to form double strands again, but with one strand from one species and one from the other. Strands with high homology will bond more tightly than those with low homology, due to more complementary nucleotide pairings. The hybrid strand is then reheated, causing more poorly matched strands to separate more easily. The temperature at which these hybrid strands separate is measured to determine how closely related species are.
What are the advantages and disadvantages of DNA-DNA hydridisation versus DNA/rRNA/protein sequencing?
DNA-DNA hybridisation is faster and cheaper, however DNA/rRNA/protein sequencing is higher resolution.
How does DNA/rRNA/protein sequencing work?
DNA is amplified using PCR then read using electrophoresis, comparing the number of differences.
What is the definition of a species?
A species that reproduces sexually can be defined by the ability of its members to actually or potentially interbreed to produce fertile offspring. Other methods of species definition must be used for those that do not reproduce sexually including morphological similarity, biochemical similarity, and sharing a common gene pool.
What is the definition of a community?
All of the populations of different species in an area at a particular time.
What is the definition of a population?
A group of organisms of the same species occupying a particular space at a particular time.
What is morphological similarity?
The development of common characteristics that make organisms recognisably similar to one another, but there are still variations (alleles) between individuals within the species.
What is biochemical similarity?
Biochemical similarity is the differences in DNA sequencing between two species. Members of the same species have much greater similarity in their DNA and amino acid sequences.
What is a gene pool?
The gene pool of a population includes all alleles of all genes of all the individuals of that population. Each species has a separate gene pool and is reproductively isolated from all other species.
How can reproductive isolation be achieved?
PREZYGOTIC FACTORS - behavioural isolation, mechanical isolation, temporal isolation, and gamete isolation
POSTZYGOTIC FACTORS - hybrid inviability, and hybrid sterility
What is behavioural isolation?
Mating behaviour is specific to individual species, not interesting to other species.