Topic 4 Flashcards

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1
Q

What is a transverse cross section?

A

Sections cut through in a structure from right angle to its length

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2
Q

What is a longitudinal cross section?

A

Sections cut along the length of a structure

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3
Q

Explain how to dissect a plant stem

A
  • Use a scalpel to cut a cross section of the stem
  • Cut as thinly as possible for better viewing under light microscope
  • Use tweezers to gently place sections in water to stop drying out
  • Transfer each section to dish containing toluidine blue O stain
  • Leave for a minute
  • Rise off the sections in water
  • Mount each one onto a slide
  • Place prepared slide under microscope
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4
Q

What colour should xylem vessels and sclerenchyma fibres look and why?

A

Blue-green, TBO stains liginin blue-green

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5
Q

What colour should phloem cells and rest of plant tissue look?

A

Pinkish purple

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6
Q

Define “tensile strength”

A

Maximum load a fibre can take before it breaks

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7
Q

Describe the tensile strength of a plant fibre experiment

A
  • Attach fibre to clamp stand
  • Hang weight from other end
  • Keep adding weights one at a time until fibre breaks
  • Record mass needed to break fibre: higher mass: higher tensile strength
  • Repeat experiment with different samples of same fibre
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8
Q

What variables should be kept constant in plant fibre experiment?

A
  • Temperature

- Humidity

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9
Q

Describe how to investigate plant mineral deficiencies.

A
  • Make up 3 nutrient broths containing all essential minerals but vary concentration of one of them in each one e.g one high calcium conc, one medium, one low
  • Split 9 test tubes into three groups and fill tubes of each group with one of the three broths
  • Take 9 seedlings of the same plant
  • Measure mass of each seedling using balance and record
  • Suspend root in nutrient broth
  • Put cotton wool inside tube opening to support seedling
  • Cover outside of test tubes in aluminium foil
  • Place all tubes near light source e.g windowsill and leave for 2 weeks
  • Top up broth to ensure continuous suspension in liquid
  • Carefully remove each plant from test tube and blot dry
  • Measure and record new masses
  • Calculate mean change in mass for each nutrient broth and any visual differences
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10
Q

Why do test tubes need to be covered in aluminium foil in investigating plant deficiencies?

A

So no light can get to nutrient broth and cause organisms e.g algae to grow

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11
Q

What type of plant should be used for investigating mineral deficiencies?

A

Germinated mung beans that are the same age

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12
Q

Describe how to test antimicrobial properties of a plant?

A
  • Take extracts from plants to investigate
  • Dry and grind each plant and soak in ethanol [antimicrobial substances: soluble in ethanol]
  • Filter off liquid with dissolved plant extract
  • Transfer bacteria grown in broth with wire inoculation loop to agar plate
  • Spread bacteria over top evenly using loop
  • Dip equally sized discs of absorbent paper in plant extracts
  • Use control disc only soaked in ethanol [ensures no antimicrobial properties in ethanol/absorbent paper]
  • Place on agar plate evenly spread out
  • Tape lid on
  • Invert
  • Incubate
  • At 25c for 24 to 48 hours
  • Where bacteria doesn’t grow are the inhibition zones - measure diameter
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13
Q

Why are bacteria incubated at 25 degrees?

A
  • Low enough to stop human harming pathogens growing

- High enough for bacteria to grow well

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