Topic 3 - DNA Flashcards
What is DNA made up of
Nucleotides
What is DNA
Strands of polymer made up of repeating nucleotides
What does a nucleotide consist of
One sugar molecule, phosphate molecule and a base
What does the sugar and phosphate molecules in the nucleotide form
Backbone
What joins to each sugar molecule
A,T,C,G
What are the complementary base pairings
A and T
C and G
What are the complementary pairs joined together with
Weak hydrogen bonds
What is the structure of a chromosome
Long, coiled up molecules of DNA found in the nucleus of eukaryotic cells
How do you extract DNA from a fruit cell
1) mash the fruit and put into a beaker that contains detergent and salt
2) filter to get a froth and big bits out
3) add ice cold alcohol
4) fish out the stringy white precipitate with a rod
Why is detergent used in extracting DMA from a fruit cell
Breaks down the cell membrane to release DNA
Why is salt used in the extraction of fruit cells
Sticks DNA together