Topic 3 - Bacterial cell differentiation Flashcards
Bacterial Development results in cells having
one of four basic Functions
Stress survival
Physiological specialisation
Cell dispersal
Symbiotic relationship
what is Myxococcus xanthus a model organism for
developmental differentiation
kinship + altruism + cooperation
type IV pili
bacterial motor systems
prokaryotic signalling
what is encoded in Myxococcus xanthus’ large chromosome
10Mb genome
200 TCS
181 transcriptional regulators
43 sigma factors
Differences between Myxococcus xanthus and E. Coli
more sigma factors - can respond to more things
kinship - can coordinate behaviour across a whole population
what does starvation lead to in Myxococcus xanthus
initiates the differentiation pathway to form a fruiting body waits till it cannot be avoided as it is reversible
starvation is detected –> ppGpp, A signalling
C signals co-ordinate the building of the fruiting body
what are fruiting bodies made up of
cells that have undergone autolysis
what are the 3 cell fates of Myxococcus xanthus differentiation
80% - autolysis, liberate nutrients
15% - spores, thick carbohydrate coat and 2N genome
5% - peripheral rods
C signals
Contact dependent signals responsible for fruiting bodies through CsgA a short chain alcohol dehydrogenase
contact dependant “signal”
produces diacylglycerol (DAG) which diffuses (the c signal) OR other theory the protein is made into smaller signal by protease and displayed on the cell surface (C signal)
what is the point of kin recognition
to stop other bacteria using the cells to form its own spores
kin recognition proteins? what happens?
TraA = recognition protein
Glycan = receptor
specific to species - the two cells must express similar alleles of TraA
when bound outer membranes may fuse share lipids etc.
what is A-signalling
aromatic amino acids initial factor in starvation response as they are costly to make
What is physiological Specialisation?
The formation/occurrence of cells with distinct and complimentary physiological features (e.g. myxobacteria fruiting bodies and cyanobacteria heterocysts)
What is Stress Survival?
The conversion/ gene switching of cells into a less metabolically active form with high resistance and don’t divide (e.g Bacillus endospores)
what is cell dispersal
motility eg. flagella, surface associated motility, or passive motility by wind, water, or animals
what is myxococcus xanthus a model organism for
they show developmental differentiation, kinship, altruism and cooperation, type IV pili, horizontal gene transfer and prokaryotic signalling processes
what is a difference between myxococcus xanthus and e. coli and what does this allow
M xanthus has more sigma factors and can therefore respond to more things and coordinate behaviour across a population
what are the three distinct fates of myxobacteria with results and %
autolysis (to liberate nutrients) 80%
spores (resistance to heat, desiccation and radiation) 15%
peripheral rods (“scout cells”) 5%
what are peripheral rod cells
stay vegetative but hardly divide
“scout cells” can initiate swarm development when they encounter nutrients
how do populations orchestrate cell movements (form chain)
C-signal (technically not a signal, it is Contact dpenedant)
what is kin recognition, what is required for it to occur
in myxobacteria, ability to transfer outer membrane proteins and lipids, involves fusion of the outer membrane
only occurs if the the cells express a similar allele of a cell surface protein called TraA
where are myxobacteria found
the soil
what are cyanobacteria
- diverse group of photosynthetic prokaryotes, found in oceans, lakes and soil
- Gram-negative photoautotrophs (fix CO2 in the Calvin cycle)
- Often show gliding motility (none have flagella)
- Lack of a source of “fixed” nitrogen (e.g. ammonium ions NH4+) results in the formation of specialised cells (heterocysts) to convert (“fix”) atmospheric N2 to ammonium ions.
what is ppGpp what can it activate
an intracellular stress response (pheromone - alarmone) which also regulates sporulation
activates regulon –> increase nutrient uptake, transcribe chaperones, reprogram metabolism
describe the ultrastructure of a bacillus endospore, what is its purpose
multiple layers of peptidoglycan - thick cortex - resistant to organic solvents and chemicals
dehydrated core - heat and radiation resistant
looses some things to protect DNA - this is the purpose of spores
how is DNA stabilised in a bacillus endospore
dipicolinic acid (DPA) (normally as Ca2+ DPA complex)
what is the difference between endosporulation and myxosporylation
in endospores the division is unequal with one mother cell and prespore cell, in myxospores the entire cell will become spherical and turn into a spore
structure of a bacillus endospore
outer coat, inner coat, cortex, core, nucleoid
instructions for diagram of endospore formation
cell can go to vegetative growth or sporulation
1. axial filament - inner membrane goes in a bit on one side (DNA pump is ensuring DNA is correctly separated)
2. membrane septum forms - mother cell, forespore
3. engulfment
4. cortex forms (mother chromosome is degraded)
5. exosporangium forms (Ca(II) DPA synthesis)
6. spore coat formed
7. spore is released
can germinate back to vegetative cell
what features make spores (bacillus endospore) resistant to chemical and physical stress
the hydrated core is resistant to heat and radiation stress
SASPs small acid soluble proteins protect DNA from heat chemicals and radiation
as does the Ca2+DPA which makes up 5-15% dry weight of the nucleoid (forms crystals)
the coat of the spore is resistant to organic solvents due to its low permeability
what signals lead to the formation of spores (+ differentiation)
A signals and unloaded tRNA - starvation
this activates the stringent response - alarmones ppGpp and ppGppp this leads to formation of resting cells such as spores
differentiation
C signal collect cells together contact dependent
Kin recognition using TraA and TraB proteins only share lipid with cells with the same alleles in TraA/TraB
sigma F
early stages of Forespore development