Topic 2: Microscopes and magnification Flashcards
Define resolution.
A measure of the microscopes ability to distinguish between two close prints on an object or between two close objects. How clear the image is.
Define magnification.
The object’s increase in size when observed with a microscope, it is expressed with a ratio.
Describe a light microscope.
A microscope that requires a might much to work either from an external source reflected by a mirror or by a built in light pointing directly at the lens. Or works by passing light through the object you’re viewing and focusing the light through the lenses which magnifies the image through light refraction. The best light microscopes can magnify up to x1500
Describe a scanning electron microscope
Its users a beam of electrons to view a magnified image. A beam of electrons is moved back and forth over a cell’s surface which creates the detail. SEMs are able to knock electrons off the object these lost electrons come together to form an image, watch due to this can be 3D
Describe a transmission electron microscope
REMs use a beam of electrons to create as magnified image. High energy electrons are used to penetrate the cell and provide details of a cell’s internal structure. The sample must be thin.
What is the structure of the cell membrane?
Consists of a phospholipid bilayer that is about 7nm thick. Hydrophilic heads form the inner and outer surfaces and the hydrophobic tails form the inside of the membrane. The structure is described as fluid mosaic model - made of many constantly moving structures within the bilayer. Cholesterol molecules are embedded between the phospholipids to prevent too much movement. Channel proteins and carrier proteins are found within the bilayer to allow large molecules and ions to be transported across. Therefore the membrane is partially permeable. Receptor proteins, glycoproteins and glycolipids are scattered through the membrane.
What is the structure of the cell membrane?
Consists of a phospholipid bilayer that is about 7nm thick. Hydrophilic heads form the inner and outer surfaces and the hydrophobic tails form the inside of the membrane. The structure is described as fluid mosaic model - made of many constantly moving structures within the bilayer. Cholesterol molecules are embedded between the phospholipids to prevent too much movement. Channel proteins and carrier proteins are found within the bilayer to allow large molecules and ions to be transported across. Therefore the membrane is partially permeable. Receptor proteins, glycoproteins and glycolipids are scattered through the membrane.
What is the function of the cell membrane?
Physical barrier - prevents the movement of foreign bodies and other molecules entering the cell.
What do the glycoproteins and glycolipids do?
Respond to insulin in liver cells resulting in the cells absorbing glucose from the bloodstream.
Establishing blood type.
Immune responses.
Responding to neurotransmitters involved in nervous responses
Name the parts of the nucleus.
Nucleolus, chromatin, nuclear envelope, nuclear pore
What is the structure of the nucleus?
Has a double membrane (nuclear envelope ), with small spaces in it (nuclear pores).
Chromatin (contains DNA and protein is scattered throughout.
Nucleolus consists of DNA, RNA, and proteins
What is the function of a nucleus?
Cousins the functions of the cell through DNA transcription. Controls gene expression, protein synthesis, storing DNA. Protein synthesis and ribosome production occurs in the nucleolus
What’s the purpose of the nuclear pores?
To allow mRNA to leave the nucleus.
What happens to the chromatin when cells divide?
Condenses into visible chromosomes
What is the structure of a ribosome?
Made of two subunits (small subunit and large subunit). Very small. Consists of ribosomal proteins and ribosomal RNA (rRNA). No membrane. Often line RER.
What’s the function of a ribosome?
Protein synthesis from mRNA during translation
What is the structure of the rough endoplasmic reticulum?
Network of channel-like structures filled with fluid linked to the nuclear membrane with tubules. Is lined with ribosomes
What is the function of the RER?
Works with the ribosomes to process and produce the 3D structures of proteins. Site of glycoprotein synthesis.
What is the structure of the smooth endoplasmic reticulum?
Very similar to RER but no ribosomes. Linked to the RER and nuclear membrane. Large surface area for rate of synthesis of lipids and order molecules.
What is the function of the SER?
Storing, synthesising, and processing lipids, phospholipids and cholesterol. In skeletal muscle cells it stores calcium ions. Within some endocrine gland it contains enzymes that detoxify harmful substances
What is the structure of the mitochondria?
Oval shaped with two membranes. The inner membrane is very folded, which forms finger like structures called cristae - increase surface area. The matrix is formed from the folding of the inner cell membrane and contains some of the enzymes involved in aerobic respiration
What is the function of the mitochondria?
Site of aerobic respiration to produce adenosine triphosphate (ATP)
What is the structure of the Golgi?
Formed of a Golgi apparatus and several small vesicles. Golgi apparatus is membrane bound and channel-like and filled with fluid. The vesicles are detached fluid filled pockets found at the edges
What is the function of the Golgi?
Processing and packaging lipids and proteins. Produces lysosomes. The lipids and proteins formed are stored in the vesicles which can transport them out of the cell through the cell membrane