Topic 12 - Energy from (electro)chemical gradients II

Question 1

Chemiosmotic hypothesis

Answer 1

All energy sources are linked to energy sinks via the PMF

Question 2

3 basic tenets of the chemiosmotic hypothesis

Answer 2

1. The respiratory and photosynthetic electron transfer chains should translocate protons.
   
   2. The ATP synthase should function as a reversible proton-translocating ATPase.
   3. Energy-transducing membranes should have a low effective proton conductance.

Question 3

Direction of proton pumping

Answer 3

From N to P

Question 4

Which side is the P side?

Answer 4

The positive side, so low pH

Question 5

What happens during uncoupling?

Answer 5

The protons are no longer used for ATP synthesis, but pass through the membrane producing heat instead of ATP

Question 6

Thermogenesis

Answer 6

Uncoupling in a controlled manner in BAT. UCP1 helps in the recovery from this state. This happens in hibernation