Topic 10 - Microbial Genomics - Clickers

Question 1

What proportion of genes in the Escherichia coli genome would you predict have ‘unknown function’?

a) 1%
b) 3%
c) 10%
d) 30%
e) 100%

Answer 1

d) 30%

Explanation: Approximately 30% of genes in the E. coli genome have functions that remain unknown, highlighting the need for further research to characterize these genes.

Question 2

If high quality sequences are more important for you than high throughput, which technology might you choose?

a) Nanopore
b) Ion torrent
c) PacBio
d) Illumina
e) Sanger

Answer 2

e) Sanger

Explanation: Sanger sequencing is ideal for obtaining high-quality sequences, though it is less suitable for high-throughput applications compared to newer technologies.

Question 3

If you already have Illumina sequences for your genome, and your genome still has gaps, how might you collect LONG reads to help bridge the gaps, without primer walking? (select all that apply)

a) Illumina
b) Ion Torrent
c) Nanopore
d) Sanger
e) PacBio

Answer 3

c) Nanopore;
e) PacBio

Explanation: Both Nanopore and PacBio technologies generate long reads that can bridge gaps in Illumina-based assemblies, complementing short-read data.