Topic 1- key concepts in biology Flashcards
What is osmosis?
Osmosis is net movement of water molecules across a partially permeable membrane from region of higher water concentration to region of lower water concentration
What is a partially permeable membrane?
it’s a membrane with small holes in it, tiny molecules (water) can pass through and bigger (sucrose) can’t
Which way do the water molecules pass through the membrane and why do they do this?
(osmosis step 1)
The water molecules pass both ways through the membrane during osmosis
(because water molecules move randomly all time)
What happens to the water molecules when there is more water molecules on one side? state which region the water molecules move to?
(osmosis step 2)
when there are more water molecules on one side of the membrane than the other there’s a net flow of water into the region with fewer water molecules
what happens to the solution in osmosis? And what happens to the water itself on what?
(osmosis step 3)
the solute solution gets more dilute, the water acts like it’s trying to equalize the concentration on either side of the membrane.
How do you calculate the percentage change in mass?
percentage change = final mass - initial mass/ initial mass x 100
What happens when the water concentration of the sucrose solutions is higher than in the potato bit?
The potato bit gains mass as water is drawn in by osmosis (hypertonic)
What happens when there is no change in mass?
The fluid inside the cylinders and the sucrose solution are isotonic (same water concentration)
What happens when the water concentration of the sucrose solutions is lower than the potato bit?
This causes the potato bit to lose water so their mass decreases (hypotonic)
What is Diffusion?
Diffusion is the net (overall) movement of particles from an area of higher concentration to an area of lower concentration
What states of matter does diffusion happen in?
Why?
Diffusion happens in both liquids & gases
because these substances are free to move about randomly
What types of molecules can diffuse in diffusion?
What types of molecules can and can’t go fit through the cell membrane?
Only very small molecules can diffuse through cell membranes
CAN-glucose, amino acids, water & oxygen
CAN’T- Starch and proteins
What is active transport? What is used in active transport?
Active transport is the movement of particles across a membrane against a concentration gradient from a area of lower concentration to an area of higher concentration using energy transferred during respiration
How is the movement particles different in active transport compared to diffusion? What does this process require?
Particles are moved up a concentration gradient rather than down
the process requires energy (diffusion = passive process)
What is calorimetry?
Food that can be burnt to see how much energy it contains
How do you calculate the energy in food? And the energy per gram of food?
energy in food (in J) = Mass of water (in g) x temp change of water (in C) x 4.2
energy per gram of food (in J/g) = energy in food (in J) / mass of food (in g)
How do you test for reducing sugars? What is a coloured precipitate?
1) Add benedict’s reagent to a sample (blue)
2) Heat it in a water bath (75 C)
3) If it’s positive–> coloured precipitate (solid particles suspended in solution)
What’s the order of precipitate concentration from low to high when benedict’s reagent is used in a sample?
blue —-> green —> yellow —-> orange —-> brick red
( low to high sugar concentration)
How does the biruet test work?
Why do you add the first solution? To make it what?
What colours does it turn if it contains & doesn’t contain proteins?
1) add potassium hydroxide to make the solution alkaline
2) then add copper (2) sulphate solution (bright blue)
3) no protein —> blue
2) protein (present) —> purple
What is biuret test is used for?
To find out if a substance contains proteins