Tools of Developmental Biology Flashcards
1
Q
PCR
A
Amplify a specific gene of interest
2
Q
DNA Microarrays
A
Can analyze differential expression of genes throughout the entire genome at once
3
Q
Western Blotting
A
Quantify protein abundance of a single protein
4
Q
Northern Blotting
A
Quantify RNA abundance
5
Q
Southern Blotting
A
Quantify DNA abundance
6
Q
Immunostaining
A
Localization of proteins within cells/organisms
7
Q
in situ hybridization
A
measures RNA expression of specific mRNAs
8
Q
RNAi
A
degrade target mRNA
9
Q
Morpholinos
A
Small oligonucleotide prevents specific mRNA from attaching to ribosome - prevents translation
10
Q
Genetic Mutagenesis
A
Forward genetics - increase (cause) random mutations
11
Q
Genetic KO
A
Reverse genetics - remove one specific gene
12
Q
Transgenics
A
Adding a new copy of a gene
