To Investigate The Effect Of Heat Denaturation On Enzyme Activity Flashcards
To which group of biomolecule do enzymes belong?
Protein
Name the part of an enzyme to which the substrate attaches.
Active site
Name the enzyme you used
Catalase
Name the substrate you used
Hydrogen peroxide
Name the product formed as a result of enzyme action
Oxygen
How did you denature the enzyme?
Boil the solution for ten minutes
Name a suitable control
No enzyme
Why must the pH be kept ocnstant?
Enzyme will be denatured otherwise
How did you keep it constant?
Use of pH 9 buffer solution
How did you know that the enzyme had been denatured?
Foam is not produced by the boiled enzyme but is for the un-boiled enzyme
Suggest one possible source of error in this investigation
3
Temperature not high enough to denature enzyme
Not heated for sufficient time to denature enzyme
No buffer added
What term is used for the substance(s) that result(s) from the action of an enzyme on its substrate?
Products
Explain the term optimum activity
Working at maximum rate
Comment on the molecular shape of enzymes.
They are folded
What role do enzymes play in metabolism?
They are catalysts - they speed up reactions
