TISSUE PROCESSING Flashcards
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convenient backup in times of power interruption, breakdown of tissue processor
manual method
mostly used tissue processing method
automatic method
dip and dunk machine
tissue transfer processor
casettes stay in just one chamber and fluids are pumped in and out
fluid transfer
in fluid transfer, specimens do not dry out. t or f
true
how many stations in processing and how much time each
12 stations each 1 hour
reagents vapors are venetd through filters or retianed in a closed loop system
fluid transfer
sample processing take how much
12 hours
tissues muct be adequately prepared and set in a ___ ready for cutting
paraffin block
alteration of tissues by stabilizing proetin so that tissue is resistant to change
fixation
fixative of choice
10% neutral buffered formalin
in fixation, your changing insolube to solube
false, soluble to insolube=le
—— fixation leads to IRREVERSIBLE tissue damage and damage of sections
suboptimal/delayed
role of fixation in tissue processing
allows thin section
protects cell from all subsequent processing
improve cell avidity for stain`
tissue fixation should be in what volume
10:1 - 20:1
time tissue is exposed to formalin
fixation time
acceptable range for fixation time
6-48 hours
t or f. most water is retained before infiltrated by wax
false: remove water
recommended reagwnt for dehydration
alcohol (ethyl alcohol/ ethanol)
how much time is dehydration
6 hours
properties of alcohol for dehydration
fast-acting, mix with water and organic
penetrates tissue easily
non toxic and non expensive
what should be added first to fatty tissues with benign neoplasms of fat before final absolute ethanol
acetone and isopropanol
why is dehydration graded
to minimize cell shrinkage and extraction of cell components
dehyeration involves —– subst of water
slow
in dehydration, volume of ethanol should not be less than 10 times volume of tissue to ensure tissue penetration
true
