Tissue Homeostasis Flashcards
What are two uses for stem cells in tissues?
-Renewing a constantly turning over tissue (like blood)
-Renewing a tissue after injury (muscle)
What are traits of stem cells?
-Immortal
-Slowly dividing
-Can give rise to fast dividing daughters (transit amplifiers)
May give rise to either a differentiated cell or an undifferentiated copy of itself
How can asymmetric divisions be induced?
Either by:
-outer environmental signals (growth factors, surrounding ecm…)
-Internal localised cell components that only end up in one daughter
What is a technique which can be used to uniquely label implanted cells.
Lightly irradiating the cells will cause unique DNA damage in each of them.
What is a niche?
The environment of a stem cell which provides factors needed for stem cell maintenance. If a daughter cell is no longer in its niche then it will differentiate.
What is the niche for Germline stem cells in drosophila?
Cap cells
What is the purpose of Bam gene in drosophila Germline stem cell differentiation?
Bam gene is activated in daughter cystoblasts produced by GSCs due to a lack of signalling received from the cap cell niche.
What causes oriented division in drosophila Germline stem cells
The spectrosome (a condensed organelle composed of spectrin) is oriented towards the cap cell niche in GSCs and inherited by the GSC daughter. Orients division towards and away from the cap cell.
What differentiated cells are present in the gut?
-Enterocyte: nutrient absorption
-Goblet cell: secretes mucus
-Enteroendocrine cell: secretes hormones
-Paneth cell: mediates microbial immunity
-Crypt base columnar (CBC) cells: express LGR5 (are stem cells)
What is the niche for Crypt base columnar cells?
Paneth cells
Which signals do Paneth cells release to allow CBC cell survival?
Wnt3
How do CBC daughters move away from the Paneth cells in order to differentiate?
Paneth cells do not divide and so form a small net in the crypt. CBCs divide symmetrically and compete for space in the crypt. Some of them get pushed away from and lose contact with the Paneth cells and so go on the become transit amplifying region cells.
How is the gut regenerated after CBC stem cells are removed?
Transit amplifying cells further toward the villus regenerate the CBC (de-differentiation?)
Organoids can also be implanted to reintroduce CBCs
What is the structure of different tissue layers in the skin?
-Undifferentiated basal cells at bottom (stem cells). These give rise to cells which divide for several rounds and then give rise to suprabasal cells.
-suprabasal cells are above, which progress toward outer surface and lay down a protein matrix coated in specialised cell membrane, these cells then enucleate and die to make a barrier
-outer layer (epidermis) is consistently lost
Out of the 3 colony types in cultured skin cells, which can sustain long term propagation?
Holoclones