tissue engineering Flashcards
what are the advantages and disadv of CaOH pulp capping?
- excellent antibacterial properties
2) induces min of dentin - low cytotoxicity
disadv:
- high soluble in oral fluids
- subject to dissolution
- presence of funnels in reparative dentin
- lack of adhesion
what are the adv and disadv of ZOE as a pulp cap?
adv:
1. reduces inflammation
disadv:
- lack of calcfic bridge formation
- release eugenol inhigh conc–>cytotoxicity
- interfacial leakage
what are the adv and disadv of corticosteroids and abx for pulp capping?
adv:
1. reduces pulp inflammation
2. vancomycin and CaOH stimulate more reparitive dentin
disadv:
1. should not be used in pts with risk from bacterimia
what are the adv and disadv of polycarboxylate cements as pulp capping material?
adv:
1. chem. bonds to tooth
disadv:
- lacks antibacterial effects
- does not stimulate calcific bridge formation
what are the adv and disadv of inert materials like ISOBUTYL CYANOACRYLATE and CaPO3
adv:
1. reduces pulp inflammation
2. stims dentin bridge formation
disadv:
1. not viable bc of lack of technique
what are the adv and disadv of collagen as a pulp cap mateiral?
adv:
1. less irritating, promotes mineralizatiopn
disadv:
1. does not help in thick dentin bridge formation
describe calcium phosphate as a pulp cap material
adv:
- helps in bridge formation with no superficial necrosis
- no inflammation
- good physical properties
disadv:
1. must perform clinical trials to evaluate this mateiral
what are the adv and disadv of hydroxyapatite as a pulp capmaterial?
adv:
- biocompatible
- scaffold for new dentin formation
disadv:
1. mild inflammation with superficial necrosis of pulp
what are the adv and disadv of CO2 lasers
adv
- formation of secondary dentin
- bacterialcidal effects
disadv
- technique sensitive
- cause thermal damage to pulp at high doses
what are the adv and disadv of GI/RMGI as a pulp cap material?
adv
- excellent bacterial seal
- fluoride release,
- CTE and modulus of elasticity similar to dentin
- good biocompatibility
disadv
- causes chronic inflammation
- lack of dentin bridge formation
- cytotoxic when in direct cell contact
- high solubility and slow setting rate
what are the adv and disadv of MTAas a pulp capping material
adv
- good biocompatiblity
- not much pulpal inflammation
- more predictable hard tissue barrier
- radiopacity
disadv
- expensive
- poor handling characteristics
- two step procedure
- high solubility
what are the adv and disadv of growth factor as a pulp capping material?
adv
- formation of osteodentin and tubular dentin
- formation of more homogenous reparative dentin
- superior to CaOH for mineralization
disadv
- high conc required
- half life is less
- appropriate dose response is required to prevent obliteration of pulp chamber
what are the adv and disadv of odontogenic meloblast associated proteins
adv
- biocomaptible
- accelerates reactionary dentin formation
- does not promote excessive teriary dentin formation
disadv:
1. only in vitro studies conducted
\2. further studies required
what are the adv and disadv of thera cal as a pulp capping material?
adv
- protect pulp
- strong physical properties
- no solubility
- radiopaque
- good Ca release
disadv
1. can affect composite shading–unesthettic
what ar ethe factors that determine the success ofpulp capping?
- remaining dentin thickness
- choice of indirect pulp capping agent
what is the purpose of cyanoacrylate
- placed over wound site
- polymerizes in the presence of moisture
- forms a PROTECTIVE COATING
what are the adv and disadv of cyanoacrylate monomer?
adv
- good biocompatilbity (only butyl and isobutyl forms)
- good tissue adherence
- biodegradable–>wound heals and it breaks down
- provides hemostasis
- bacteriostatic
- easy to apply (to small surfaces)
- shortens operation time
disadv:
- difficult to apply to large wounds
- is toxic in methyl form
what is n-butyl-2-cyanoacrylate and what is it used for?
a tissue adhesives, not cytotoxic –> can be used instread of stitches and parrier from food/debris during the healing period
what are the absorbable sutures?
PGA
PDO
catgut
what are the non-absorbable sutures?
silk
polyester
nylon
what is an important benefit of an alloplast?
do not pose the risk of transmitting disease
what is guided tissue regeneration?
undesired cells excluded from repopulating a defects or injury site by placing a physical barrier; desriable cells can enter
describe cell induction as tissue regeneration?
growth and differentiation factors are injected; can also create gene vectors and cause growth/diff factors to be produced endogenously
describe cells as a scaffolding matrix as a strategy for tissue engineering
preformed scaffolds are seeded with cells from a pt; grown in vitro –> increase in # of cells–> start producing matrix; with enough growth, implanted back into pt, cells grow and develop into tissues; scaffold resorbs and leaves no former prescence
strategries for tx engineering?
- injection of cells – right at site of injury
- guided tissue regeneration
- cell induction –
- cells in a scaffold matrix
what does SIBLINGs stand for
small integrin bindin ligand, N-linked glycoprotein
- includes bone sialoprotein (BSP),
- osteopontin (OPN)
- dentin sialophosphoprotein (DSPP)
- dentin-matrix protein 1 (DMP-1)
- matrix extrecellular pohosphoprotein (MEPE)
- noncollagenous
- mediate cell adhesion
what does allotropic mean?
that an element can exist in different physical forms
what materials are used in perio, and for what purpose?
used to promote healing
- ZOE
- Non-eugenol (zinc polyacrylate, resin)
cyanoacrylate molecule
