tissue culture

Question 1

who introduced concept of totipotency

Answer 1

T. H. Morgan

Question 2

what’s micropropagation

Answer 2

plantlets with unchanged qualities of parent by using a small part

Question 3

father of tissue culture

Answer 3

Gottlieb Haberlandt

Question 4

how many rooms are present in tissue culture lab?

Answer 4

3 rooms

Question 5

tissue culture is also called

Answer 5

in-vitro culture

Question 6

explant is -

Answer 6

plant part used in tissue culture

Question 7

explant is sterilized w -

Answer 7

sodium hypochlorite, Br water and 70% alcohol

Question 8

main translocated sugar for plants -

Answer 8

sucrose

Question 9

what is used to maintain the homogeneity and semisolid nature of culture medium?

Answer 9

agar

Question 10

pH of culture medium is

Answer 10

5.5 - 5.8

Question 11

condition for autoclaving equipment and medium

Answer 11

121`C 15 atm for 20 mins

Question 12

knife, forceps etc is made germ fre by

Answer 12

ethyl alcohol

Question 13

who used the term genetic engineering first

Answer 13

jack williamsonet

Question 14

genetic engineering first used in which book

Answer 14

dragon’s island

Question 15

father of genetic eng.

Answer 15

paul berg

Question 16

which enzyme is called molecular knife

Answer 16

endonuclease

Question 17

which enzyme is used for synthesis of DNA using PCR?

Answer 17

taq polymerase (thermostable DNA polymerase)

Question 18

who first observed plasmid in E> coli

Answer 18

Ladenberg

Question 19

which plasmid has resistance power against 6 antibiotic

Answer 19

R6

Question 20

what type of plasmid causes disease to host bodies

Answer 20

virulence pplasmid